用于禽流感H5亚型检测的压电免疫蛋白芯片的研制  

A Piezoelectric Immunity Protein Chips for Determination of H5 Subtype Avian Influenza(AIV) Viruses

作　　者：刘毅新[1] 刘仲明[1] 王弘[2] 孙远明[2] 

机构地区：[1]广州军区广州总医院,广东广州510010 [2]华南农业大学食品学院,广东广州510642

出　　处：《中国医学物理学杂志》2007年第2期119-121,共3页Chinese Journal of Medical Physics

基　　金：广东省重大科技专项资助(编号:2005A2090101)

摘　　要：采用聚乙烯亚胺(PEI)粘附、戊二醛(Glu)交联的方法,将禽流感H5抗原固定于石英晶体的金电极表面,制成压电蛋白芯片。结果显示,抗原最佳固定浓度为0.761 mg/mL,该芯片用于检测浓度范围为0.1243 mg/mL～1.243 mg/mL的禽流感H5血清时,呈线性相关,相关系数0.9704,且阳性样品的响应值与阴性样品的噪声值之比大于2,可用于定性定量检测。H5 subtype avian influenza viruses antigen was fixed on gold electrod surface of the quartz crystal to make piezoelectric immunity protein chips by polyethylenimine adhesion and glutaraldehyde cross-linking method. Results showed that the effective immobility concentration of antigen was 0.761 mg/mL, the perfect resonant range of the sensor was between 0.1243 mg/mL and 1.243 mg/mL and the correlated coefficient was 0.9704. The positive serum was 2 times higher than negative serum and the piezoelectric immobility protein chips could be used for qualitative and quantitative detection.

关 键 词：禽流感H5亚型 戊二醛交联法 压电石英晶体 免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]