Possible Role of Histone H1 in the Regulation of Furin-dependent Proprotein Processing  

作　　者：Jinbo HAN Jianxin GU Chengwu CHI 

机构地区：[1]Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China [2]Gene Research Center, Medical Center of Fudan University, Shanghai 200032, China [3]Institute of Protein Research, Tongji University, Shanghai 200092, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第3期173-180,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Basic Research Program of China(2004CB719904)

摘　　要：Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitors of furin, a mammalian proprotein convertase. However, its role in the regulation of furin-dependent proprotein processing remains unclear. Here we report that histone H1 efficiently blocks furin-dependent pro-von Willebrand factor （pro-vWF） processing in a dose-dependent manner. Coimmunoprecipitation and immunofluorescence studies confirmed that histone H1 could interact with furin, and the interaction mainly took place on the cell surface. We noted that histone H1 was released from cells undergoing necrosis and apoptosis induced by H2O2. Our findings suggested that histone H1 might be involved in extracellular and/or intracellular furin regulation.Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitors of furin, a mammalian proprotein convertase. However, its role in the regulation of furin-dependent proprotein processing remains unclear. Here we report that histone H1 efficiently blocks furin-dependent pro-von Willebrand factor （pro-vWF） processing in a dose-dependent manner. Coimmunoprecipitation and immunofluorescence studies confirmed that histone H1 could interact with furin, and the interaction mainly took place on the cell surface. We noted that histone H1 was released from cells undergoing necrosis and apoptosis induced by H2O2. Our findings suggested that histone H1 might be involved in extracellular and/or intracellular furin regulation.

关 键 词：FURIN histone H1 pro-vWF proprotein processing 

分 类 号：Q512.7[生物学—生物化学]