川芎嗪对宫内生长受限幼鼠生长发育及脑损伤GAP-43蛋白表达的影响  

Effect of Tetramethylpyrazine on the Growth,Development and the GAP-43 Protein Expression in the Hippocampus of Fetal Growth Restriction Rats

作　　者：洪莹[1] 王燕[1] 张元珍[1] 王德刚[2] 

机构地区：[1]武汉大学中南医院妇产科,湖北武汉430071 [2]湖北省利川市人民医院妇产科,湖北利川445400

出　　处：《武汉大学学报（医学版）》2007年第2期143-146,I0002,共5页Medical Journal of Wuhan University

基　　金：湖北省科技攻关项目(编号:2003AA301C38)

摘　　要：目的:研究钳夹子宫血管致宫内生长受限幼鼠(FGR)脑神经相关生长蛋白-43(GAP-43)的表达及川芎嗪对FGR幼鼠及脑海马GAP-43蛋白表达的影响。方法:通过钳夹子宫血管30 min建立FGR模型,测出生当天及生后7 d假手术组、模型组、川芎嗪低剂量组及川芎嗪高剂量组幼鼠的体重、脑重、肝重,应用免疫组化方法观察川芎嗪对FGR幼鼠脑海马GAP-43蛋白表达的影响。结果:出生当天,模型组幼鼠的体重、脑重、肝重、GAP-43蛋白的表达均明显低于假手术组(P<0.01);川芎嗪低剂量组体重、脑重、肝重、GAP-43蛋白的表达与模型组无显著差别(P>0.05),川芎嗪高剂量组体重、脑重、肝重、GAP-43蛋白的表达明显高于模型组(P<0.01)。生后7 d,4组幼鼠体重无明显差异(P>0.05),但模型组的脑重、肝重及GAP-43蛋白的表达仍显著低于假手术组及川芎嗪高剂量组(P<0.05);同川芎嗪低剂量组无显著差异。结论:足够剂量的川芎嗪对钳夹子宫血管致FGR幼鼠脑组织有明显的保护作用。Objective： To investigate the effect of the tetramethylpyrazine on the expression of GAP-43 protein in the hippocampus of fetal growth restriction （FGR） rats. Methods： Animal models of FGR were established by clamping the uterine vessel of pregnant rats for 30 minutes at gestational 17 d after pre-treatment of tetramethylpyrazine. Body, brain and liver were weighted at birth and on the seventh day after birth. GAP-43 proteins were also measured by immunohistochemistry in the hippocampus. Results： Statistical analysis demonstrated a significant decrease in the weight of fetus, brain, liver and the expression of GAP-43 protein in model group compared with the sham operated group （all P〈0. 01）. No significant difference of the weight of fetus body, brain, liver and the expression of GAP-43 protein was found between the low dose group and the model group（all P〉0.05）. The expression of GAP-43 protein was increased obviously in high dose group （P〈0.01）compared with the model group. The weight of brain and liver of the fetus and the expression of GAP-43 protein in model group were less or lower than in sham operated group and high dose group （all P〈0.05） on day 7 after birth. There is no significant difference between model group and low dose group in the above indices（all P^0.05）. Conclusion： Higher dose of tetramethylpyrazine has protective effects on brain tissue of FGR rats.

关 键 词：宫内生长受限 大鼠 脑 川芎嗪 神经相关生长蛋白-43 

分 类 号：R714.12[医药卫生—妇产科学]