退变前后兔腰椎间盘细胞凋亡及蛋白多糖的比较  

Apoptosis and Proteoglycans Changes in the Degeneration of Lumbar Intervertebral Disc of Rabbits

作　　者：张振兴[1] 徐振华[1] 牛光峰[1] 

机构地区：[1]武汉大学中南医院骨科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2007年第2期169-171,共3页Medical Journal of Wuhan University

摘　　要：目的:动态观察细胞凋亡及蛋白多糖在兔腰椎间盘退变前后的改变。方法:建立动静力失衡性腰椎间盘退变模型,分别用Tunel法和Bitter法检测造模5月后椎间盘细胞凋亡程度及蛋白多糖量的变化。结果:与对照组比较,模型组细胞凋亡数明显升高(P<0.01)。结论:退变椎间盘细胞凋亡数目增多及蛋白多糖含量的减少可能在椎间盘退变过程中起重要作用。Objective： To study the cell apoptosis and the content of proteoglycans in the degenerated intervertebral disc. Methods： Sixteen rabbits were divided into an operated group and a control group. The operated group was incised through posterior approach along spinous process and resected the supraspinal and interspinal ligament and the posterior half of the zygapophysial joint between the second and the sixth lumbar spine. The control group was only incised in the skin. The corresponding discs were removed five months later, and the apoptosis cells were examined and analyzed using TUNEL and Vidas analysis system. Results： The apoptosis cells in the control group was less than that in the operated group（P〈0.01）. Also the content of proteoglycans was less in the disc of model group than in that of the control group （P〈0. 01）. Conelusion： There are much more apoptotic cells but less proteoglycans in the degeneration of lumbar intervertebral discs. Apoptosis and proteoglycans changes might play an important role in the pathogenesis of disc degeneration.

关 键 词：椎间盘 退行性改变 凋亡 蛋白多糖 

分 类 号：R681[医药卫生—骨科学]