电针足三里激活胆碱能抗炎通路抗大鼠感染性休克  

Study on Electro-acupuncture at "Zusanli" Points Against Septic Shock of Rats Through Activating the Cholinergic Anti-inflammatory Pathway

作　　者：袁翔[1] 李建国[1] 黄越[1] 杜朝晖[1] 贾宝辉[1] 周青[1] 

机构地区：[1]武汉大学中南医院ICU,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2007年第2期203-206,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金资助项目(编号:2004ABA161);湖北省卫生厅科研基金资助项目(编号:JX2B37)

摘　　要：目的:观察电针足三里穴对感染性休克大鼠平均动脉压(MAP)及生存指标的影响,探讨电针足三里穴对感染性休克是否具有保护作用及其发挥作用的机制。方法:雄性SD大鼠63只按随机数字表法分为假休克组、休克组、足三里组、非经非穴组、迷走神经切断组、迷切电针组、M受体拮抗组、N受体拮抗组和N受体-α7亚单位拮抗组9组,每组7只。采用盲肠结扎加穿孔(CLP)法制备感染性休克动物模型,于模型制备成功后即刻,以2 V和3 Hz的强度持续电针刺激双侧足三里穴,于刺激开始前颈总动脉置管连续监测MAP,另取40只大鼠随机分为假休克组、休克组、足三里组和迷切电针组4组,处理同上相应各组,除此不作其它干预,观察生存状况。结果:休克组MAP持续降低,生存时间显著缩短,各时间点的生存率均显著降低。非经非穴组与休克组比较上述指标无显著改变(均为P>0.05)。而足三里组在电针刺激后MAP明显高于休克组,生存时间及相应时间点的生存率明显提高。电针足三里前离断迷走神经,则上述作用完全消失。电针足三里前静脉注射阿托品,不影响保护效应,但静脉注射六烃季胺或-αBGT,则可完全抵消上述保护效应。结论:电针足三里对感染性休克动物具有保护作用。这种保护作用可能是通过激活胆碱能抗炎通路,拮抗全身性炎性反应来实现的。Objective： To investigate the effects and mechanisms of electro-acupuncture （EA） at Zusanli points on the change of mean arterial （MAP） and survival indices in the rats with septic shock （SS）. Methods： Sixty-three SD rats were randomly divided into nine groups equally： sham shock group, SS group, Zusanli points group, non-acupoints group, bilateral cervical vagotomy group, vagotomy and electroacupuncture （EA） group, muscarinic antagonist group, nicotinic antagonist group and α-BGT group. The Zusanli points were electro-acupunctured with constant voltage （2 V, 3 Hz） for 30 minutes immediately after the shock models were established. Before stimulation, a blood pressure transducer was implanted into the carotid artery for continuously monitoring of MAP. Another 40 rats were randomly divided into sham shock group, SS group, Zusanli points group and non-acupoints group. Survival time and survival rate were observed in these groups. Results： In SS group, the MAP, survival time and survival rate was decreased markedly. The differences of MAP, survival time and survival rate were not significant between non-acupoints group and SS group. EA Zusanli points, as well as muscarinic antagonist, significantly reversed the marked hypotension, and increased the survival time and survival rate. Conclusion： Electro-acupuncture at Zusanli points might have a protective effect on rats with septic shock through activating the cholinergic anti-inflammatory pathway.

关 键 词：电针 足三里 感染性休克 胆碱能抗炎通路 

分 类 号：R605[医药卫生—外科学]