Study on the Supramolecular Interaction of β-Cyclodextrin with Gemfibrozil by Spectrofluorimetry and Its Analytical Application  

作　　者：黄辉 刘芳 贾宝秀 徐克花 陈蓁蓁 唐波 

机构地区：[1]College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, Shandong 250014, China

出　　处：《Chinese Journal of Chemistry》2007年第3期337-342,共6页中国化学（英文版）

基　　金：Project supported by the Program for New Century Excellent Talents in University （No. NCET-04-0651）, the National Natural Science Foundation of China （Nos. 20335030 and 20575036） and the Important Project of Natural Science Foundation of Shandong Province of China （No. Z2003B01）.

摘　　要：The supramolecular interaction of gemfibrozil with β-cyclodextrin （β-CD） was studied by spectrofluorimetry. The mechanism of the inclusion was discussed by spectrofluoremetry, infrared spectrum and ^1H NMR spectrum. The results showed that a 1 ： 1 （β-CD ： gemfibrozil） complex was formed with an apparent association constant of 3.844 × 10^3 L·mol^-1. Based on the enhancement of the fluorescent intensity of gemfibrozil, a spectrofluorimetric method for the determination of gemfibrozil in bulk aqueous solution in the presence of β-CD was developed. The linear range was 3.30 ng·mL^- 1 -6.00 ug·mL^-1 with the detection limit of 0.980 ng·mL^-1. There was no interference from the excipients normally used in tablet composition and the serum main compositions. The proposed method was then successfully applied to the determination of gemfibrozil in capsules and serum.The supramolecular interaction of gemfibrozil with β-cyclodextrin （β-CD） was studied by spectrofluorimetry. The mechanism of the inclusion was discussed by spectrofluoremetry, infrared spectrum and ^1H NMR spectrum. The results showed that a 1 ： 1 （β-CD ： gemfibrozil） complex was formed with an apparent association constant of 3.844 × 10^3 L·mol^-1. Based on the enhancement of the fluorescent intensity of gemfibrozil, a spectrofluorimetric method for the determination of gemfibrozil in bulk aqueous solution in the presence of β-CD was developed. The linear range was 3.30 ng·mL^- 1 -6.00 ug·mL^-1 with the detection limit of 0.980 ng·mL^-1. There was no interference from the excipients normally used in tablet composition and the serum main compositions. The proposed method was then successfully applied to the determination of gemfibrozil in capsules and serum.

关 键 词：GEMFIBROZIL Β-CYCLODEXTRIN supramolecular interaction SPECTROFLUORIMETRY 

分 类 号：R972.6[医药卫生—药品] R917[医药卫生—药学]