大黄素对大鼠肝移植排斥反应的抑制作用  

Inhibitory effect of emodin on rejection following orthotopic liver transplantation in rats

作　　者：林胜璋[1] 徐贤绸[1] 童洪飞[1] 余耀军[1] 游涛[1] 

机构地区：[1]温州医学院附属第二医院外科,温州325003

出　　处：《中华器官移植杂志》2007年第3期147-149,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金（N030572395）

摘　　要：目的探讨大黄素预防大鼠肝移植后排斥反应的效果。方法以Wistar大鼠为供者，SD大鼠为受者，建立大鼠原位肝移植模型。（1）将肝移植大鼠模型随机分为5组，其中3组分别腹腔注射大黄素2．0、10．0和50．0mg·kg^-1·d^-1，另外2组分别给予CsA（10．0mg·kg^-1·d^-1）和生理盐水（0．5ml／d）作为对照，观察各组大鼠的存活时间。（2）另取肝移植大鼠模型，按给予大黄素时间的不同随机分为4组，分别于术前8d至手术当天、术前4d至术后4d、术后第1～8天及术后第8～16天腹腔注射大黄素50．0mg·kg^-1·d^-1，观察各组大鼠的存活时间。（3）另取肝移植大鼠模型，随机分为4组，分别给予CsA、大黄素以及CsA＋大黄素，CsA的用量为10．0mg·kg^-1·d^-1，大黄素的用量为50．0mg·kg^-1·d^-1，各组于手术当天至术后第8天连续给药，停药后1周处死大鼠，观察各组大鼠移植肝组织病理学变化，根据Banff分级方案确定排斥活动指数。结果接受大黄素10．0和50．0mg·kg^-1·d^-1腹腔注射的大鼠，术后存活时间分别为（25．1±2．1）d、（27．1±7．3）d，明显长于生理盐水对照组（P〈0．01），使用50．0mg·kg^-1·d^-1者的存活时间与CsA对照组相比，差异无统计学意义（P〉0．05）。术前8d至手术当天、术前4d至术后4d用药者，术后受者存活时间分别为（15．3±1．6）d、（15．8±1．9）d，二者的存活时间明显短于术后第1～8天用药者的（25．3±3．7）d和术后第8～16天用药者的（23．1±3．8）d（P〈0．01）。采用CsA与大黄素联合用药者，移植肝的排斥活动指数为2．11±0．78，明显低于单用CsA或大黄素者（P〈0．05），单用CsA者（6．56±0．88）与单用大黄素者（6．33±1．00）比较，排斥活动指数的差异无统计学意义。结论大黄素对大鼠肝移植后的排斥反应有抑制作用。Objective To evaluate the preventive effect of emodin on rejection following orthotopic liver transplantation （OLT） in rats. Methods The Wistar→SD OLT models were established. （1） A total of 45 rats were divided randomly and equally into 5 groups： emodin group A was treated with emodin at dose of 2. 0 mg·kg^-1·d^-1 intraperitoneally after operation; emodin group B, emodin at dose of 10.0 mg·kg^-1·d^-1; emodin group C, emodin at dose of 50. 0 mg·kg^-1·d^-1 ; CsA group, CsA at dose of 10. 0 mg·kg^-1·d^-1 ; control group, normal saline at dose of 0. 5 ml/d.The survival of recipients were observed. （2） A total of 36 rats were divided randomly and equally into 4 groups according to different time of emodin administration： group A, B, C and D respectively. The survival of recipients was observed. （3） A total of 36 rats were divided randomly and equally into 4 groups： group A was treated with emodin; group B, CsA; group C, emodin and CsA; group D, control group. Rejection active index （RAI） was confirmed after observing the pathologic change of transplanted liver in recipients. Results The survival in emodin groups B and C and CsA group was 25. 11 ± 2. 09,27. 11 ± 7. 27 and 27. 67 ± 5.66 days, respectively, which was longer remarkably than in control group （P〈 0. 01）. The survival of groups C and D was longer notably than in control group. There was significant difference in the RAI of transplanted liver between group B, C and group A and between group B, C and Group D. Conclusion Emodin can suppress acute rejection following OLT in rats.

关 键 词：大黄素 肝移植 移植物排斥 抑制作用 

分 类 号：R686[医药卫生—骨科学]