银屑病皮损中Toll样受体4、NF-κBp65、HSP60的表达  

The expression of Toll-like receptor 4, NF-κBp65 and HSP60 in psoriatic lesions

作　　者：颜兰香[1] 李浪[2] 赵娟[3] 谭琦[4] 

机构地区：[1]中南大学湘雅二医院皮肤科,长沙410011 [2]中南大学湘雅医院 [3]湖南省马王堆医院 [4]重庆医科大学附属儿童医院

出　　处：《中华皮肤科杂志》2007年第3期149-151,共3页Chinese Journal of Dermatology

摘　　要：目的 研究Toll样受体4（TLR4）、NF-κBp65、HSP60在银屑病患者皮损中的表达水平及其与银屑病严重程度的关系。方法 采用S-P免疫组化法检测30例进行期寻常性银屑病皮损及10例正常皮肤石蜡标本中的TLR4、NF-κBp65、HSP60的表达，对其在皮损中的表达水平进行相关性分析，并将结果与PASI评分进行相关性分析。结果 与正常人对照组相比，寻常性银屑病皮损角质形成细胞中的TLR4、NF-κBp65、HSP60的表达明显上调，差异有统计学意义（P〈0．05）。TLR4、NF-κBp65与HSP60表达水平与患者PASI评分之间均存在正相关（P〈0．01）。银屑病患者TLR4、NF-κBp65、HSP60的表达水平间均呈正相关（P〈0．05）。结论 TLR4、NF-κBp65、HSP60的表达上调可能与银屑病发病及严重程度有关。HSP60可能与TLR4／NF-κBp65信号转导途径有关。Objective To investigate the expressions of Toll-like receptor 4 （TLR4）,nuclearfactor-κB （ NF-κB ） p65 and heat shock protein 60 （ HSP60 ） in progressive psoriasis vulgaris and their correlation with disease severity in psoriatic patients. Methods Paraffin-embedded tissue samples were prepared from the lesions of patients （ n = 30 ） with progressive psoriasis vulgaris and from normal control skin （ n = 10 ）, then were analyzed by SP immunohistochemical technique for the expression of TLR4, NF-κBp65 and HSP60. Correlation analysis was performed among the expression levels of three factors as well as between the levels and disease severity. Results The expressions of TLR4, NF-κBp65 and HSP60 were significantly higher in the lesions than those in the controls （ t= 7.33, 8.14, 3.53, respectively, all P〈 0.05 ）. The expressions of TLR4, NF-κBp65 and HSP60 positively correlated individually with the psoriasis area and severity index （ PASI ） scores in patients （ r = 0.908, 0.732, 0.927, respectively, all P 〈 0.01 ）. Positive correlation was also found between the expression of any two factors （ TLR4, NF-κBp65 or HSP60 ） in the lesions （ all P 〈 0.05 ）. Conclusions The up-regulation of TLR4, NF-κBp65 and HSP60 expressions may be related to the pathogenesis and severity of psoriasis. HSP60 may be involved in the TLR4/NF-κB signal pathway.

关 键 词：银屑病 NF-ΚB 监控蛋白60 TOLL样受体4 

分 类 号：R686[医药卫生—骨科学]