生存素在恶性黑素瘤中的表达及与VEGF和PCNA的关系  

Expression of survivin and its correlation with VEGF and PCNA in malignant melanoma

作　　者：陶娟[1] 涂亚庭[1] 熊芬[2] 李延[1] 刘业强[1] 林云[1] 张晓冰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉 430022 [2]川北医学院附属医院皮肤科

出　　处：《中华皮肤科杂志》2007年第3期164-166,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目（30500437）

摘　　要：目的 探讨人恶性黑素瘤组织中凋亡抑制蛋白生存素的表达，及其与恶性黑素瘤临床特征、血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）表达的关系。方法 免疫组化方法检测36例人恶性黑素瘤和30例色素痣组织中生存素、VEGF和PCNA蛋白表达。结果 ①生存素在恶性黑素瘤和色素痣中均有表达，但在恶性黑素瘤中表达明显高于色素痣（P〈0．01）。②在恶性黑素瘤组织中有30例表达VEGF（阳性率83．3％），36例表达PCNA（阳性率100％），而色素痣组织不表达VEGF和PCNA。③生存素的阳性表达与恶性黑素瘤患者的发病年龄、性别、淋巴结转移均无相关性（P〉0．05），但其表达与VEGF和PCNA的表达密切相关（P〈0．01）。结论 生存素可能参与恶性黑素瘤的增殖和与VEGF相关的肿瘤血管生成。Objective To detect the expression of an apoptosis inhibitor, survivin, in human malignant melanoma （ MM ）, and its relationship with the clinical features of, as well as the expression of vascular endothelial growth factor （ VEGF ） and proliferative cell nuclear antigen （ PCNA ） in MM. Methods Tissue sections from 36 cases of MM and 30 pigmented nevi （ as control ） were examined with immunohistochemical technique and morphological quantitative analysis for the protein expressions of survivin, VEGF and PCNA. Results The expression of survivin was detected in both MM and pigmented nevi, but its level was higher in MM than that in pigmented nevi （t=3.87, P〈0.01）.Ofthe36MMcases, 30 83.3%） were positive for VEGF, and 36 （ 100% ） for PCNA, whereas neither was detected in pigmented nevi. The expression level of survivin did not correlate with the clinical features of MM patients, including age, gender, tumor size and metastasis （ P 〉 0.05 ）, but closely correlated with VEGF and PCNA expression in MM （ both P 〈 0.01 ）. Conclusion Survivin might take part in the growth of and VEGF- related tumor angiogenesis in MM.

关 键 词：血管内皮生长因子类 增殖细胞核抗原 黑色素瘤 生存素 

分 类 号：R686[医药卫生—骨科学]