生存素和端粒酶逆转录酶在皮肤肿瘤中的表达  

Expression of survivin and hTERT in skin tumors

作　　者：吴艳[1] 林云[1] 刘厚君[1] 李家文[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022

出　　处：《中华皮肤科杂志》2007年第3期167-169,共3页Chinese Journal of Dermatology

摘　　要：目的 研究皮肤肿瘤凋亡抑制基因生存素与人端粒酶逆转录酶（hTERT）的表达及相互关系。方法 免疫组化SP法检测17例鳞状细胞癌（鳞癌）、21例基底细胞癌（基癌）、19例鲍恩病、25例脂溢性角化病和13例正常人皮肤组织中生存素表达水平：原位杂交法检测上述组织中hTERT mRNA的表达水平：分析皮肤肿瘤中生存素与hTERT mRNA表达的相关性。结果 ①在鳞癌、基癌、鲍恩病中生存素及hTERT mRNA表达的阳性率均较正常人皮肤中显著增加：②脂溢性角化病中生存素表达的阳性率与正常人皮肤组织中比较差异有统计学意义．但hTERT mRNA表达的阳性率与正常人皮肤组织中比较差异无统计学意义；③鳞癌、基癌、鲍恩病中生存素阳性率及hTERT mRNA阳性率之间均呈显著正相关。脂溢性角化病中两者阳性率之间无显著相关性。结论 生存素与皮肤肿瘤发病关系密切，在鳞癌、基癌、鲍恩病中生存素与hTERT mRNA的表达具有一定的相关性。Objective To investigate the expression of survivin and human telomerase reverse transcriptase （ hTERT ） mRNA as well as their relationship with each other in skin tumors. Methods Tissue samples were resected during operation from 17 cases of squamous cell carcinoma, 21 cases of basal cell carcinoma, 19 cases of Bowen＇s disease, 25 cases of seborrheic keratosis and 13 normal human controls. The expression of survivin protein and hTERT mRNA was measured by SP immunohistochemical technique and in situ hybridization, respectively, in above samples. Results The positivity rates of survivin protein and hTERT mRNA were significantly higher in squamous cell carcinoma, basal cell carcinoma and Bowen＇s disease than those in normal controls （all P 〈 0.01 ）, and positively correlated with each other in the three tumors （r= 0.79, P〈 0.001; r= 0.79, P〈 0.001; r= 0.57, P= 0.011 ） but not in seborrheic keratosis. In seborreic keratosis, the positivity rate of survivin （P 〈 0.01 ）, rather than hTERT mRNA （P 〉.0.05 ）, was significantly increased compared with that of normal controls. Conclusion The expression of survivin seems to be correlated with that of hTERT mRNA in squamous cell carcinoma, basal cell carcinoma and Bowen＇s disease, which may contribute to the oncogenesis of skin tumors .

关 键 词：癌 基底细胞 癌 鳞状细胞 BOWEN病 角化病 脂溢性 生存素 端粒酶逆转录酶 

分 类 号：R686[医药卫生—骨科学]