Fmoc-Asn(Trt)-OH与Wang树脂的合成研究  

Synthesis of Fmoc-Asn(Trt)-Wang Resin

作　　者：宓鹏程[1] 朱颐申[1] 张琪[1] 韦萍[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《氨基酸和生物资源》2007年第1期21-24,共4页Amino Acids & Biotic Resources

摘　　要：研究了Fmoc-Asn(Trt)-OH与Wang树脂的酯化反应工艺。探讨了反应策略、溶剂体系、投料比、反应时间以及反应温度等条件对手工合成Fmoc-Asn(Trt)-Wang树脂反应的影响。并用微波辅助方法与常规方法进行了对比。实验结果表明了采用HBTU/HOBt/DIEA方法的连接效率最高。对反应的优化结果为:NMP/DCM体积比为1:7,体系、摩尔比为3:1,每次反应时间4 h,温度40℃。微波对本酯化反应影响不大。The esterification process of Fmoc- Asn（Trt） -OH and Wang resin was studied by sonu pnasc synthesis. The effects of reaction strategy, solution, molar ratio, reaction time and reaction temperature on the yield of Fmoc- Asn（Trt） -Wang resin were investigated and the results of microwave -assisted synthesis and manual synthesis were compared. The results indicated that the HBTU/HOBt/DIEA strategy was the best method and the optimum reaction conditions for the esterification were set at temperature 40℃, molar ratio 3 ： 1, reaction time 4h using NMP/DCM（ 1：7 ,v/v） solution . The esterification process was hardly influenced by microwave radiation.

关 键 词：固相多肽合成(SPPS) Wang树脂 Fmoc—Asn(Trt)-OH 微波 

分 类 号：TQ425.9[化学工程]