同种异体近交系小鼠原位气管移植模型的建立  

Establishment of an orthotopic tracheal transplantation model with inbred mice

作　　者：赵晋波[1] 李小飞[1] 刘锟[1] 周勇安[1] 梁志波[1] 程庆书[1] 

机构地区：[1]第四军医大学唐都医院胸腔外科,陕西西安710038

出　　处：《第四军医大学学报》2007年第6期540-543,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(39970707);陕西省自然科学基金(99SM37)

摘　　要：目的:建立近交系小鼠同种异体原位气管移植模型.方法:供受体体质量配对后,C57BL/6和BALB/c小鼠随机分为2组,每组各10对,A组为C57BL/6(供体)→BALB/c(受体)组,B组BALB/c(供体)→BALB/c(受体)组,昆明种小鼠10对作为C组即KM(供体)→KM(受体)组.利用颈前肌肉及周围组织被覆移植气管提供血供建立同种异体小鼠原位气管移植模型.使用HE染色以及扫描电镜对同种异体小鼠原位气管移植模型进行评估,观察比较上皮积分、淋巴细胞计数以及无核软骨细胞/全部软骨细胞等指标.结果:A,B组全部存活,C组存活6只.A,C组移植气管均发生一定程度的排斥反应,B组无排斥反应发生.A,B,C三组上皮积分分别为:1.094±0.120分,2.872±0.059分及0.995±0.444分;无核软骨细胞数/骨细胞总数比值分别为:(27.22±1.09)%,(10.60±1.01)%及(31.40±6.43)%;淋巴细胞计数分别(20.18±1.31)个/HPF,≤5个/HPF及(22.55±4.87)个/HPF.A,B组及C,B组各项指标相比均有统计学意义,而A,C组相比则差异无统计学意义;且C组各数值离散程度均大于A组.结论:成功建立了C57BL/6(供体)→BALB/c(受体)小鼠原位气管移植模型.近交系及封闭群模型均能反应移植气管的各种病理生理变化,但封闭群模型稳定性较近交系差.AIM： To establish an orthotopic tracheal transplanration model with .inbred mice. METHODS： Weight-matched inbred mice with different germlines were randomly assigned to 2 experimental groups ： A, C57BL/6 （donator）→ BALB/c （ receptor）（n=10 pairs）; B, BALB/c（donator）→BALB/c（receptor） （ n = 10 pairs）. Weight-matched Kunming （ KM ） mice were assigned as a control group C ： KM （donator） → KM （receptor） （n = 10 pairs ）. The orthotopic tracheal transplantations were performed according to the designed groups and the transplanted trachea was coated with the anterior cervical muscles for revascularization. Tracheal graft rejection was monitored by daily clinical airway assessment, histological examination using HE staining and scanning electron microscopy. RESULTS： All the mice in group A and B were living until they were sacrificed, while 6 mice kept alive in group C. Tracheal autografts in group A and C had notable rejection reaction, but group B did not. In the 3 groups, the epithelial scores were 1. 094 ± 0. 120,2. 872 ± 0.059 and 0. 995 ±0.444; the numbers of lymphocyte were （20.18 ± 1.31） / HPF, 〈5/HPF and（ 22.55 ±4.87） /HPF; the ratios of anuclear chondrocyte/osteocyte were （27.22 ± 1.09）% , （ 10. 60 ±1.01 ） % and （31.40 ± 6.43 ） %. Various cellular indexes such as the epithelial score, the number of lymphocyte and the ratio of anuclear chondrocyte/osteocyte in group A and C showed marked statistical difference when compared with those of group B, but no statistical difference was found when group A was compared with group C. CONCLUSION： A C57BL/6 （donator） → BALB/c （receptor） orthotopic tracheal transplantation medel has been established successfully. Various pathophysiological changes in thetracheal transplantation would well be reflected in both the inbred and closed population mice. The inbred model seems to be more stable than the closed population.

关 键 词：气管移植 小鼠 近交系 疾病模型 动物 

分 类 号：R562.1[医药卫生—呼吸系统]