内毒素体外诱生人单核细胞分泌PGF1α／TXB_2的研究  

Research on the Secretion of PGF_1α/TXB_2 in Human Monocytes Induced by Endotoxin in Vitro

作　　者：赵琪[1] 崔乃强[1] 吴咸中[1] 李济坤 周文洛 王民宪[1] 

机构地区：[1]天津市南开医院

出　　处：《中国中西医结合外科杂志》1996年第3期173-175,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：分离健康人外周血单核细胞，行短期体外培养后，以大肠杆菌内毒素刺激单核细胞，放免法测定细胞培养上清液中前列腺素Ｆ１α血栓素Ｂ２含量。结果表明内毒素体外能诱生人外周血单核细胞合成ＰＧＦ１α／ＴＸＢ２，内毒素刺激量为１０μｇ／ｍｌ时单核细胞合成ＰＧＦ１α／ＴＸＢ２最旺盛，刺激２小时左右合成速率达到高峰，随后下降。Supernantant PGF1α & TXB2 Lvels of endotoxin-induced adult human monocytes were measured by the method of radioimmunoassay.It was observed that endotoxin could improve the monocytes PGF1α and TXB2 secretion in vitro.The data showed that the concentration of PGF1α and TXB2 secreted by monocytes reached a peak level when the final concentration of endotoxin was 10μg/ml and the stimulated time lasted to 2 hours.

关 键 词：单核细胞 内毒素 前列腺素F1Α 血栓素B2 

分 类 号：R631.302[医药卫生—外科学]