不同培养基对四倍体刺槐叶片不定芽诱导研究  

Study on the Shoot Regeneration of Teraploid Black Locust Leaf in Different Culture Mediums

作　　者：燕丽萍[1] 夏阳[1] 梁慧敏[1] 王太明[1] 詹伟 柴传华[1] 

机构地区：[1]山东省林业科学研究院,济南250014 [2]日照大沙洼林场

出　　处：《山东林业科技》2007年第1期23-24,共2页Journal of Shandong Forestry Science and Technology

摘　　要：以四倍体刺槐不同着生节位大田叶片为试材,接种于3种不同培养基进行培养,诱导不定芽,使其形成再生植株。结果表明:以尚未充分展开复叶为最佳外植体,MS Salt+B5Vitamin+1.8 mg/L NAA+1.1mg/L6-BA培养基为最佳诱导培养基,愈伤组织诱导率、芽分化率、苗增值系数最高,分别为96.7%、93.1%、9.1倍。A research was conducted using leaves Teraplold Black Locust as explants in different culture mediums to establish a highly efficient regeneration system . The result indicated that The optimal culture medium is MSsahs ＋ B5 vitamins ＋ 1.8 mg/L NAA ＋ 1. lmg/L6- BA. callus inductivity, bud＇s differentiation rate, seedling＇s increment coefficient is the highest, 96.7%, 93.1%, 9.1 times; the young tender 1st location of knots or nodes leaf for is the best explant, the bud differentiation percentage reaches as high as 90%, 3rd, the 5th location of knots or nodes leaf bud differentiation percentage respectively is 43.3% and 0%.

关 键 词：四倍体刺槐 离体叶片 不定芽 

分 类 号：S685.99[农业科学—观赏园艺]