Quantitative Estimates of Outcrossing Rates in a Natural Population of Caldesia grandis (Alismataceae)  

作　　者：CHEN Jinming Gituru Wahiti Robert WANG Qingfeng 

机构地区：[1]College of Life Sciences, Wuhan University, Wuhan 430072,Hubei, China [2]Botany Department, Jomo Kenyatta University of Agricultureand Technology, P. O. Box 62000-00200, Nairobi, Kenya

出　　处：《Wuhan University Journal of Natural Sciences》2007年第2期337-342,共6页武汉大学学报（自然科学英文版）

基　　金：Supported by the State Key Basic Research and Develop-ment Plan (G2000046805);the National Natural Science Foundation of China (30370098, 30570111)

摘　　要：Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.

关 键 词：ALISMATACEAE clonal plant Caldesia grandis mating system random amplified polymorphism DNA（RAPD） 

分 类 号：Q943[生物学—植物学]