同时产甲烷反硝化颗粒污泥中微生物群落结构  

Microbial community structure in simultaneous methanogenesis and denitrification granular sludge

作　　者：孙寓姣[1] 左剑恶[1] 陈莉莉[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《中国环境科学》2007年第1期44-48,共5页China Environmental Science

基　　金：中国博士后基金项目(2005038073)

摘　　要：在UASB成功运行同时产甲烷反硝化小试基础上,针对反应器内颗粒污泥,通过构建古菌和细菌的16SrDNA基因文库、RADAR遗传变异分型和测序比对等技术进行了微生物系统发育关系和群落结构分析.结果表明,在颗粒污泥的古菌中,产甲烷髦毛菌和产甲烷杆菌是主要菌群,随机选出的88个古菌克隆子,这两类古菌的16SrDNA序列分别占古菌总量71.59%和22.73%;而颗粒污泥中细菌的多样性要高于古菌,低GC革兰氏阳性菌和ε变型菌纲分支的细菌是细菌的主要菌群,在随机选出的133个细菌克隆子中,这2类细菌的16SrDNA序列分别占细菌总量的49.62%和12.03%.Based on the successful performance in a lab-scale UASB reactor with the function of simultaneous methanogenesis and denitrification, the phylogenetic relationships and community structure of the microbe in the granule were analyzed by using the technologies of construction of 16S rDNA gene libraries RADAR, and sequence blast of Archaea and Eubacteria, In the Archaea of granule, the methanosaeta and methanobacteria were the dominant species, in the 88 random selected Archaea clones, the 16S rDNA sequence of these two species were 71.95% and 22.75% of the total amount of Archaea respectively. The diversity of Eubacteria in the granule was higher than the Archaea. The low GC Gram positive bacteria and ε protebacteria were the dominant Eubacteria species in the granule, and in the random selected 133 Eubacteria clones, the 16S rDNA of these two species were 49.62% and 12.03% of the total amount of Eubacteria. The ecological composition of the microbial community in the simultaneous methanogenesis and denitrification granule could be understood, and the theoretical direction could be supplied to the operation of practical process.

关 键 词：同时产甲烷反硝化 颗粒污泥 16S rDNA基因文库 群落结构 

分 类 号：X703.5[环境科学与工程—环境工程]