家蚕微孢子虫(浙江株)α-微管蛋白基因部分片段的克隆及系统发育分析  

Clone and Phylogenetic Analysis of Partial α-Tubulin Gene of Nosema bombycis(Zhejiang Strain)

作　　者：张海燕[1] 万淼[1] 费晨[1] 钱永华[1] 鲁兴萌[2] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]浙江大学动物科学学院蚕蜂研究所,杭州310029

出　　处：《蚕业科学》2007年第1期49-56,共8页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB-121003);教育部博士点基金项目(编号20050335009);国家自然科学基金项目(编号30471311)

摘　　要：基于微孢子虫分类学地位的争议,通过克隆家蚕微孢子虫(Nosema bombycis)的α-Tubulin基因,利用BioEditor将核苷酸序列翻译成氨基酸序列,并从NCBI中收集不同物种的α-Tubulin基因,用CLUSTALX(1.81)、Mega2以及在线生物信息学软件CLUSTALW(1.83)分析序列并构建系统发育树。结果显示:家蚕微孢子虫及其它微孢子虫与真菌聚为一类,且微孢子虫以一个独立群与接合菌(zygomycetes)的噬虫霉属(Entomophaga)、耳霉属(Conidiobolus)关系最近,与子囊菌(ascomycetes)、担子菌(basidiomycetes)、壶菌(chytrids)及其它接合菌互为姐妹群。The taxonomy position of microsporidia has caused intense argument in the world. A partial sequence of α-Tubulin gene of Nosema bombycis was cloned and translated to amino acids by BioEditor software. Several α-Tubulin genes of different species were collected from Genbank of NCBI. By using ClustalX （1.81）, Mega2 software and online ClustalW（1.83） two phylogenetic trees were constructed. The result indicated that microsporidia was a member of fungi group. As a single sub-group microsporidia showed the nearest relationship with Entomophaga and Conidiobolus which were zygomycetes, and as a sister group to ascomycetes, basidiomycetes, chytrids and other zygomycetes.

关 键 词：家蚕微孢子虫 α-微管蛋白基因 系统发育树 

分 类 号：S884[农业科学—特种经济动物饲养]