蓖麻蚕血液胰凝乳蛋白酶抑制剂种类的调查  

Investigation of Chymotrypsin Inhibitor Types From Haemolymph of the Eri Silkworm,Philosamia cynthia ricini

作　　者：赵秀振[1] 赵巧玲[1] 唐顺明[1] 沈兴家[1] 徐安英[1] 张国政[1] 郭锡杰[1] 

机构地区：[1]中国农业科学院蚕业研究所农业部家蚕生物技术重点开放实验室,镇江212018

出　　处：《蚕业科学》2007年第1期133-137,共5页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB-121000);江苏省自然科学基金前期预研重大项目(编号BK2004206);江苏省高技术研究计划(农业)(编号BG2005302)

摘　　要：胰凝乳蛋白酶抑制剂(CI)为昆虫体内重要的调控因子,是昆虫体内免疫系统的重要组成部分。通过非变性聚丙烯酰胺凝胶电泳和活性染色方法,对20个蓖麻蚕品种血液CI的种类进行了调查。在碱性聚丙烯酰胺凝胶电泳中检测到7种CI,定名为E-CI1、E-CI2、E-CI3、E-CI4、E-CI5、E-CI6、E-CI7;在酸性聚丙烯酰胺凝胶电泳中检测到2种CI,定名为E-CIa、E-CIb。对同一蓖麻蚕品种不同发育时期血液CI的调查显示,CI 5的活性有随5龄期经过而递增的趋势。Chyrnotrypsin inhibitors （CIs） in insect haernolymph play a major function of proteinase controlling which is essential to life, and they are also important factors in innate immune system, By using acid and alkaline Nativepolyacrylamide gel electrophoresis followed by activity staining, the polyrnorphism of CIs in twenty strains of eri silkworm, Philosamia cynthia ricini, were investigated. Seven types of CIs, designated E-CI1, E-CI2, E-CI3 ,E-CI4, E- CI5, E-CI6 and E-CI7 by alkaline Native-PAGE, and two types of CIs, designated E-Cla and E-CIb by acid NativePAGE were detected, respectively. Investigation of CIs activity of different developmental stages from the same strain revealed that CIs gradually increase along with the development of larvae in the fifth instar,

关 键 词：蓖麻蚕 血液 胰凝乳蛋白酶抑制剂 非变性聚丙烯酰胺凝胶电泳 

分 类 号：S885.2[农业科学—特种经济动物饲养]