不同时程应激对大鼠行为、免疫和交感神经系统反应的影响  

The Dynamic Changes of Behavioral, Immune and Sympathetic Responses to Chronic Stress in Rats

作　　者：王玮文[1,2] 邵枫[3] 林文娟[1,2] 

机构地区：[1]中国科学院心理研究所心理健康重点实验室 [2]中国科学院心理研究所脑-行为研究中心,北京100101 [3]北京大学心理学系

出　　处：《心理学报》2007年第2期292-298,共7页Acta Psychologica Sinica

基　　金：国家自然科学基金项目资助(30500158;30670707);中国科学院创新工程(KSCX2-2-03)项目。

摘　　要：研究不同时程情绪应激诱导的大鼠行为、免疫和神经内分泌反应的动态变化及其相互关系。实验采用一种在程序性饮水的固定时间点不确定性给予大鼠空瓶刺激诱发其情绪反应的应激模式,测定不同时程(14,21和28天)应激对大鼠行为、交感神经系统反应、体液免疫功能及体重增长的影响。结果表明,情绪应激导致大鼠攻击行为显著增加,且在整个应激过程中情绪应激组大鼠的攻击行为都被稳定地诱导;不同时程的情绪应激均导致大鼠特异性抗OVA抗体水平明显降低,且降低的程度类似;在情绪应激的第14天和28天应激组大鼠血中去甲肾上腺素水平明显高于对照组,但后者升高的水平明显低于前者。此外,14天应激明显抑制大鼠的体重增长,但随着应激时程的延长,体重增长逐渐恢复。这些结果表明,随着应激时程的延长,大鼠行为、免疫和神经内分泌反应的适应性改变存在时程差异,有助于进一步了解应激诱发的各种反应间的复杂关系。Previous studies have shown that emotional stress induced by randomly giving an empty water bottle to rats during watering periods for 14 consecutive days activated both the hypothalamic - pituitary - adrenal axis and the sympathetic nervous system, leading to the increased blood levels of corticosterone and norepinephrine. It also elicited attacking and exploring behaviors, suppressed the level of specific anti - ovalbumin IgG antibody production which was negatively related to norepinephrine level. However, three day empty bottle presentation had no significant effect on anti - OVA antibody production. These results suggest that the duration of stressors determines the consequences of body reactions induced by stress, at least, humoral immune responses. In order to examine whether the duration of stress exerts an accumulative effect or an adaptive effect on body reactions, the present study was designed to investigate the dynamic changes in behavioral, immune and neuroendocrinc responses of rats that were subjected to chronic emotional stress with different durations. Male Wistar rats were divided into four groups： （1） emotional stress （induced by randomly giving an empty water bottle to rats at set watering time）（n = 19）; （2） physiological stress （given neither empty water bottles nor water during the same watering periods as for emotional stress group） （n = 19）; （3） control 1 （allowed free access to water during watering periods）（n = 15）; and （4） control 2 （given water ad libitum during the whole experimental periods）（n = 15）. Behavioral changes, humoral immune responses and the reaction of sympathetic nervous system were measured and compared during different days（14,21 and 28 days） of stress. The behaviors observed included attacking, exploring and grooming, Norepinephrin levels in plasma were determined using high - performance liquid chromatography. Serum levels of IgG antibodies to Ovalbumin were determined by enzyme linked immunosorbent assay. It w

关 键 词：情绪应激 体液免疫 去甲肾上腺素 行为 

分 类 号：B845[哲学宗教—心理学]