氯胺酮对新生大鼠神经功能和海马谷氨酸受体及转运体表达的影响  

Effects of ketamine on neuronal behavior and expression of hippocampus glutamatergic receptor and transporter in neonatal rats

作　　者：蒯建科[1] 姚立农[1] 于代华[1] 杨永慧[1] 张凤林[1] 柴伟[1] 孙绪德[1] 彭德民[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安市710038

出　　处：《临床麻醉学杂志》2007年第3期218-220,共3页Journal of Clinical Anesthesiology

基　　金：陕西省社会发展科技公关项目(2003G-K10)

摘　　要：目的观察新生大鼠氯胺酮麻醉后神经行为和海马谷氨酸N-甲基-D-天冬氨酸(NM-DA)R2受体及谷氨酸转运体蛋白Ⅰ(GLAST)的远期改变。方法新生1周Wistar大鼠40只,随机均分为四组,每组10只。K100组腹腔注射氯胺酮100mg.kg-1.h-1;K50组腹腔注射氯胺酮50mg.kg-1.h-1,均持续麻醉6h;生理盐水组(N组)给予与氯胺酮同体积生理盐水;空白对照组(C组)不予任何干预。麻醉后21d进行旷场实验和Morris水迷宫实验,海马标本行谷氨酸NMDAR2受体及转运体GLAST免疫组织化学染色,共聚焦显微镜观察并采集图象,ImageJ图象处理软件分析荧光半定量OD值。结果各组大鼠在旷场中央格的停留时间及穿越的方格数差异无统计学意义。水迷宫测试K100组、K50组的逃逸潜伏期均明显高于C组和N组(P<0.05),而探索时间短于C组和N组(P<0.05),K100组和K50组之间差异无统计学意义。麻醉处理明显增加NMDAR2和谷氨酸转运体GLAST的表达,K100组和K50组之间差异无统计学意义。结论氯胺酮麻醉新生大鼠可引起神经功能和海马谷氨酸NMDAR2受体及转运体GLAST的远期改变。Objective To Observe the long-term changes of neuronal behavior and glutamatergic NMDAR2 and GLAST in neonatal rats after ketamine administration. Methods Fourty neonatal 1w Wistar rats were randomly divided into four groups with 10 rats each. The rats in groups K100 and K50 administered intraperitoneally ketamine 100 mg · kg^-1 · h^-1 and 50 mg · kg^-1 · h^-1 for 6 h respectively,in group CON were given no any treatment, in group NS administered equal volume NS for experimental control. Open field and Morris water Maze were tested at the 21 day after anesthesia. Meanwhile, immunohistochemical techniques were used to observe the changes of NMDAR2 and GLAST at hippocampus. Results The retention time in central check and the locomotion of experimental groups were not significantly different from that in control group. The escape latency of group K100 and group K50 was longer than that of groups CON and NS （P〈0.05） and the spatial probe test shorter than that of groups CON and NS （P〈0.05）, which was not significantly different between group K100 and K50. Keta mine increased the expression of NMDAR2 and GLAST in the hippocampus compared with group CON and group NS（P〈0.05）, which was not significantly different between group K100 and K50. Conclusion Ketamine induces long-term changes of neuronal behavior and glutamatergic NMDAR2 and GLAST expression in neonatal rats.

关 键 词：谷氨酸受体 谷氨酸转运体 海马 氯胺酮 

分 类 号：R96[医药卫生—药理学]