中华根瘤菌NL的筛选与鉴定  

Study on Isolation and Characterization of Sinorhizobium NL

作　　者：曾晓希[1,2] 胡岳华[1] 柳建设[1] 胡培磊[1] 周洪波[1] 

机构地区：[1]中南大学资源加工与生物工程学院 [2]湖南工业大学包装印刷学院,湖南株洲412007

出　　处：《湖北农业科学》2007年第2期180-182,共3页Hubei Agricultural Sciences

基　　金：国家自然基金创新研究群体科学基金项目(50321402)

摘　　要：从吉林某菜园土样中筛选到菌株NL,用该菌对伊利石进行浸矿脱硅试验,测得接种NL的溶液中的可溶性硅的浓度比对照液中的增67.83%,说明该菌能促进伊利石中硅的溶解。从菌株NL的培养特征、形态大小、生理生化测试和16S rDNA序列的比对分析等方面进行了鉴定,其16S rDNA序列与GenBank中的中华根瘤菌属Sinorhizobium sp.S1-5(AY505134)的相似性达99.62%。试验结果表明菌株NL属于中华根瘤菌(Sinorhizobium)。The strain NL isolated from garden soil in Jilin was carried to remove silicon from illite. The result showed the content of silicon in the medium which was inoculated with NL was 67.83% higher than that of the no-inoculating medium and indicated the strain NL can remove silicon from illite. The strain was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The result showed that the 16S rDNA similities of the strain NL and Sinorhizobium sp. S1-5 （AY505134）was more than 99.62% in Genbank. Based on the above results, the strain NL was identified as Sinorhizobium sp.

关 键 词：中华根瘤菌 16S RDNA 鉴定 土壤 

分 类 号：Q939[生物学—微生物学] S154.3[农业科学—土壤学]