PCR法构建小鼠α-1,3-GT基因沉默载体研究  

Construction of Murine α-1,3-GT Gene Silencing Vector by PCR

作　　者：刘中华[1] 谷习文[2] 郑新民[2] 魏庆信[2] 赵献军[1] 肖红卫[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《湖北农业科学》2007年第2期187-189,共3页Hubei Agricultural Sciences

基　　金：湖北省科技厅资助项目(2004TZ01);国家自然科学基金项目(30671495)

摘　　要：在已报道的shRNA序列前加上人类H1启动子,设计一段沉默小鼠α-1,3-GT基因的siRNA序列,针对该序列分段设计引物,完全采用PCR法将其连接起来,然后把该序列克隆到pGEM-T载体中,构建出针对小鼠α-1,3-GT基因的特异性沉默载体,为进一步获得α-1,3-GT基因沉默小鼠,构建异种器官移植用小鼠模型奠定了基础。Through putting human H1 promoter before the shRNA reported, a siRNA sequence that would silence a-1,3-GT gene in murine was designed, a series of primer segments were subsequently devised and synthesized, and joined by PCR. A vector that could silence the murine a-1,3-GT gene was acquired by cloning the connected product into the pGEM-T vector. This would help us to constitute an important foundation for further development of a-1,3-GT knock down mice and for mouse model used for xenotransplantation research.

关 键 词：小干扰RNA 基因沉默 α-1 3-半乳糖苷转移酶 

分 类 号：Q785[生物学—分子生物学] Q786