离子对色谱法测定棉隆原药的含量  

Determination of Purity of Dazomet by Paired Ion Chromatography

作　　者：高春香[1] 孙伶[1] 王蕾[1] 

机构地区：[1]沈阳化工学院测试中心,辽宁沈阳110142

出　　处：《沈阳化工学院学报》2007年第1期22-24,共3页Journal of Shenyang Institute of Chemical Technolgy

摘　　要：研究测定棉隆原药质量分数的相关问题.采用反相离子对液相色谱法测定棉隆原药的质量分数,优化流动相的组成及离子对试剂的种类和用量.最终选定的色谱操作条件是:C18反相色谱柱为分析柱,甲醇-磷酸二氢钾溶液-HEPS体系为流动相,紫外可变波长检测器的检测波长为280 nm.得到外标定量工作曲线的回归方程:y=38.71X+0.444 2,相关系数为0.999 6,线性范围为0.08-1.2 g/L,加标回收率为99.81%,相对标准偏差为0.469%.该方法具有良好的重现性.A reversed-phase high performance paired ion chromatography method has been developed to determining the 3,5-dimethyltetrahydro-1,3,5-thiadiazine-2-thione （DMTT） content in dazomet. The composition of the mobile phase and the kind of the ion-pair have been selected. As a result, the optimum experimental condition were established as follows：a C18 column as a anilysis column, the mobile phase consisted of methanol and potassium dihydrogen phosphate solution and sodium alkyl sulfate., UV 280 nm as detection wavelength. In the above case, the linear correlation coefficient was 0. 999 6 and the dynamic linear range was 0.08-1.2 g/L by the method of external standard calibration, the average recoveries was 99.81%, and the relative standard deviation was 0. 469 %. This means there was a good linear correlation between the peak areas and concentration of DMTT and there was a good repetition rate also.

关 键 词：棉隆原药 质量分数 反相高效液相色谱 离子对法 外标定量 

分 类 号：O658[理学—分析化学]