血必净注射液对脓毒症大鼠组织肿瘤坏死因子-α及凝血功能的影响  

Effects of Xuebijing injection(血必净注射液) on tissue tumor necrosis factor-α and blood coagulation parameters in septic rats

作　　者：李银平[1] 乔佑杰[1] 武子霞[2] 姚咏明[3] 于燕[3] 吴瑶[3] 

机构地区：[1]天津市天和医院,天津300050 [2]天津中医药大学,天津300193 [3]解放军总医院第一附属医院(原解放军第三四医院)全军烧伤研究所,北京100037

出　　处：《中国中西医结合急救杂志》2007年第2期104-107,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：天津市卫生局中西医结合科研资助项目(2005088)

摘　　要：目的：探讨血必净注射液对脓毒症大鼠凝血功能及组织肿瘤坏死因子-α（TNF-α）的影响。方法：采用盲肠结扎穿孔术（CLP）制备脓毒症动物模型。96只健康Wistar大鼠按随机数字表法分为正常对照组、假手术组、模型组和血必净治疗组，后两组分别于CLP后0．5、12、24、36、48和60h经大鼠阴茎背静脉注射生理盐水或血必净注射液4ml／kg，分别于CLP后2、8、24、48和72h5个时间点各处死8只动物。各组大鼠取腹主动脉血检测血浆凝血酶原时间（PT）、活化部分凝血酶原时间（APTT）及纤维蛋白原（Fbg）含量；取肝、肺组织测定TNF-α蛋白水平。结果：CLP后2h模型组动物肝、肺组织TNF-α蛋白水平迅速升高（P均〈0．01），8h达到高峰，分别为正常对照组的2．57倍（P〈0．01）和4．77倍（P〈0．01）。血必净注射液治疗后可显著降低肝、肺组织TNF-α蛋白水平（P〈0．05或P〈0．01），于CLP后24h均恢复至伤前正常范围。模型组CLP后2～72h。PT、APTT有不同程度的缩短，血浆Fbg水平显著降低（P〈0．05或P〈0．01）；血必净治疗组CLP后2～24hPT、APTT均较模型组明显延长（P〈0．05或P〈0．01），血浆Fbg含量回升。结论：血必净注射液可显著降低脓毒痒大鼠组织TNF-α蛋白水平，防止凝血功能异常，从而有效防止脓毒症的发生发展。Objective： To investigate the effects of traditional Chinese medicine Xuebijing injection （血必净注射液） on tissue tumor necrosis factor-α （TNF -α） expression and blood coagulation parameters in septic rats. Methods： Wistar rats were subjected to sepsis induced by cecal ligation and puncture （CLP）. Ninety - six healthy rats were randomly divided into four groups： normal group, sham operation group, CLP model group, and Xuebijing - treated group. The two latter groups were given respectively intravenous injection of normal saline or Xuebijing injection with the dose of 4 ml/kg at 0.5, 12, 24, 36, 48 and 60 hours after the establishment of CLP model. Eight rats were sacrificed at 2, 8, 24, 48 and 72 hours post -CLP in the two latter groups. Prothrombin time （PT）, activated partial thromboplastin time （APTT）, and fibrinogen （Fbg） levels were determined. Tissue TNF -α protein levels in liver and lung tissues were also measured at various intervals. Results： TNF -α protein levels in liver and lung tissues were significantly increased at 2 hours after establishment of CLP model compared with those of the normal group （both P〈0.01）, and they were 2.7：7 -fold （P〈0.01） and 4.77 -fold （P〈0. 01） of baseline values at 8 hours post -CLP, respectively. However, treatment with Xuebijing injection markedly reduced TNF-α protein levels both in liver and lung tissues （P〈0.05 or P〈0.01）, recovering to normal baseline values at 24 hours post -CLP. In CLP model group, PT and APTT were significantly shortened, and plasma Fbg levels were significantly decreased at 2- 72 hours （P〈0.05 or p〈0. 01 ）, and they were markedly improved in the Xuebijing- treated group at 2 - 24 hours （P 〈0.05 or P 〈 0.01 ）. Conclusion ： Xuebijing injection can inhibit tissue TNF-α protein synthesis and release in vital organs in rats with sepsis, thereby it may prevent the development of blood coagulation dysfunction and severe sepsis.

关 键 词：脓毒症 血必净注射液 肿瘤坏死因子-Α 凝血功能 

分 类 号：R278[医药卫生—中医学]