饮水氯化消毒副产物MX诱导人胚肝细胞氧化应激及其与DNA损伤的关系  

Oxidative stress of human derived fetal hepatocytes induced by product of chlorinated drinking water(MX)

作　　者：周利红[1] 邹亚玲[1] 来瑞平[1] 范冠宇[1] 刘爱林[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系

出　　处：《卫生研究》2007年第2期149-151,共3页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No20577012)

摘　　要：目的研究饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2（5氢）-呋喃酮（MX）对体外培养的人胚胎肝细胞（L-02细胞）氧化应激的诱导。方法MX设10、30、100和300μmol／L4个暴露浓度，二甲基亚砜（DMSO，10ml／L）为溶剂对照，将L-02细胞染毒24小时后，检测L-02细胞内脂质过氧化产物丙二醛（MDA）、还原型谷胱甘肽（GSH）和8羟基脱氧鸟苷（8-OHdG）的含量。结果与溶剂对照相比，30、100和300μmol／L的MX能明显增加L02细胞MDA含量（P〈0．05，P〈0．001，P〈0．001），100、300μmol／L的MX使L02细胞GSH水平显著性降低（P〈0．001，P〈0．001）、8-OHdG含量显著性增加（P〈0．05，P〈0．01）。在MX0～300μmol／L浓度范围内，L-02细胞的MDA含量与8-OHdG含量呈正相关（r=0．767，P〈0．01）：GSH水平与8-OHdG含量呈负相关（r=0．761，P〈0．01）。结论MX可诱导L-02细胞氧化应激，使其脂质过氧化反应增强、抗氧化作用降低、DNA氧化损伤增加。MX引发的脂质过氧化反应和抗氧化力下降是DNA氧化损伤的影响因素之一。Objective To study the oxidative stress induced by 3-chloro-4-（ dichloromethyl）-5-hydroxy-2 [ 5H ]- furanone （MX） （a product of chlorinated drinking water） in human derived fetal hepatocytes （L-02） in vitro. Methods L-02 cells were treated at the doses of 10, 30, 100 and 300μmol/L of MX for 24h. Malondialdehyde （MDA）, reduced glutathione （GSH） and 8-hydroxydeoxyguanosine （8-OHdG）, the representative of antioxidative molecules and the marker of DNA oxidative damage respectively, were detected in L-02 cell treated by MX. Dimethyl sulfoxide （DMSO） was used as solvent control. Results （ 1 ） The content of MDA was significantly increased in L-02 cells etreated by MX at the doses of 30, 100, 300μmol/L in comparison with solvent control. （2） The level of GSH was significantly decreased and the level of 8-OHdG was significantly increased in L-02 ceils treated by MX at the concentration of 100, 300μmol/L in comparison with solvent control. （3） There was a striking positive association between MDA content and 8-OHdG level （ r = 0.767, P 〈 0.01） and a negative association between GSH level and 8-OHdG level （ r = 0.761, P 〈 0.01） in L-02 cells treated by MX at the doses from 0 to 300μmol/L. Conclusion MX could induce oxidative stress in L-02 cells including increases of lipid peroxidation and DNA oxidative damage, and weakened effect of antioxidation. DNA oxidative damage in L-02 cells might be associated with lipid peroxidation and the weakening of antioxidation induced by MX.

关 键 词：3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮 氧化应激人胚肝细胞DNA损伤 饮水卫生 

分 类 号：R123.61[医药卫生—环境卫生学]