Phylogenetic Reconstruction of the Family Acrypteridae (Orthoptera: Acridoidea) Based on Mitochondrial Cytochrome b Gene  

基于Cyt b基因的网翅蝗科系统发生重建(英文)

作　　者：霍光明[1,2] 蒋国芳[2] 孙正莉[2] 刘殿锋[2] 张雅林[1] 吕林[1] 

机构地区：[1]植保资源与病虫害治理教育部重点实验室(西北农林科技大学),西北农林科技大学昆虫博物馆,杨凌712100 [2]南京师范大学生命科学学院,江苏省生物资源技术重点实验室,南京210097

出　　处：《Journal of Genetics and Genomics》2007年第4期294-306,共13页遗传学报（英文版）

基　　金：This work was supported by National Natural Sciences Foundation of China (No. 30160015).

摘　　要：Sequences from the mitochondrial cytochrome b gene （Cyt b） were determined for 25 species from the superfamily Acridoidae and the homologous sequences of 19 species of grasshoppers were downloaded from the GenBank data library. The purpose was to develop a molecular phylogeny of the Acrypteridae, and to interpret the phylogenetic position of the family within the superfamily Acridoidea. Phylogeny was reconstructed by Maximum-parsimony （MP） and Bayesian criteria using Yunnanites coriacea and Tagasta marginella as outgroups. The alignment length of the fragments was 384 bp after excluding ambiguous sites, including 167 parsimony informative sites. In the fragments, the percentages of A ＋ T and G ＋ C were 70.7% and 29.3%, respectively. The monophyly of Arcypteridae is not supported by phylogenetic trees. Within the Arcypteridae, neither Arcypterinae nor Ceracrinae is supported as a monophyletic group. The current genus Chorthippus is not a monophyletic group, and should be a polyphyletic group. The present results are significantly different from the classification scheme of Arcypteridae, which is based on morphology.研究测定了蝗总科25种蝗虫的线粒体Cyt b部分序列,并从GenBank中下载了19种蝗亚目昆虫的Cyt b基因相应序列片段。本文目的是要建立网翅蝗科的系统发育关系并说明网翅蝗科在蝗总科中的分类地位。以瘤锥蝗科的云南蝗Yunnanites coriacea和长额橄蝗Tagasta marginella作为外群,用MP法和贝叶斯法重建系统发生树。比对后的序列长度是384 bp,包括167个简约信息位点。A+T平均含量为70.7%,C+G 平均含量29.3%。分子系统树表明:网翅蝗科并不是一个单系群。网翅蝗亚科和竹蝗亚科并非单系群。现存的雏蝗属并非单系群,应该是多系群。分子系统学研究结果和传统的基于形态特征的网翅蝗科分类体系有很大的不同。

关 键 词：CAELIFERA ARCYPTERIDAE Cyt b MONOPHYLY SUBFAMILY 

分 类 号：Q963[生物学—昆虫学] Q961