包膜糖蛋白序列分析鉴定丙型肝炎病毒宫内感染  

INTRA-UTERINE INFECTION OF HEPATITIS C VIRUS IDENTIFIED BY SEQUENCING A SEGMENT OF ENVELOPE GLYCOPROTEIN

作　　者：郝飞[1] 李梦东[1] 陈国致[1] 

机构地区：[1]第三军医大学附属西南医院

出　　处：《中华肝脏病杂志》1996年第2期74-77,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金

摘　　要：目的从分子水平鉴定丙型肝炎病毒（ＨＣＶ）宫内感染的可能性，为确定ＨＣＶ垂直传播提供更直接的依据。方法分别用免疫分析法和逆转录－聚合酶链反应检测婴儿和母亲的血清抗ＨＣＶ和ＨＣＶＲＮＡ，对母婴ＨＣＶＲＮＡ阳性的血清作ＨＣＶ包膜糖蛋白Ｅ２高可变区（Ｅ２ＨＶ）序列分析和比较。结果１１例ＨＣＶ感染的母亲所生的婴儿有６例生后脐血和静脉血抗ＨＣＶ阳性，５例于１～５个月阴转，另１例持续阳性达１３个月；５例于生后ＨＣＶＲＮＡ阳性，３例于１～６个月自然转阴，２例持续阳性分别达９个月和１３个月；对２例持续ＨＣＶＲＮＡ阳性的婴儿和其母亲Ｅ２ＨＶＲ序列分析发现，生后母婴间的氨基酸同源性高达９３．３３％，而２例母亲间同源性６２．２２％。结论丙型肝炎可以发生母婴传播，并可能为宫内感染。To explore the possibility of the intra-uterine infection of hepatitis C virus(HCV) at the molecuiar level and to provide a more direct evidence which identifies the existence of vertical transmission of HCV. Methods Anti-HCV and HCV RNA in sera from mothers and their babies were analysed by enzyme linked immunosobent assays and reverse transcription-polymerase chain reaction(RT-PCR) respectively. The hypervariable region of envelope gbcoprotein E2 in HCV RNA positive sera from two mother-infant palrs was sequenced and compared. Results Among 11 babies with their mothers infected with HCV,six were shown to be positive for anti HCV in both cord blood and venous blood at birth. Anti HCV disappeared in 5 babies after 1～5 months and persisted in only one baby for 13 months.HCV RNA was found in both cord blood and venous blood from 5 babies at birth. Among them, 3 bables got a serum conversion of HCV RNA after 1～6 months and 2 were constantly positive with HCV RNA for 9 months and 13 months respectively. The homologous rate of E2HV amino acid sequences between mother and her baby was 93.33%, 62.22%, Between two mothers conclusion This study indicate that HCV could be clearly transmitted from mother to infant, possibly occurring in utero.

关 键 词：丙型肝炎病毒 母婴传播 包膜糖蛋白 序列分析 

分 类 号：R512.630.1[医药卫生—内科学]