几种不同免疫程序制备抗弓形虫高免血清的比较  

Comparative Study on High Titer Immune Serum Produced by Different Immune Programs

作　　者：江涛[1] 龚大春[2] 陈声国[1] 聂浩[1] 姚宝安[3] 赵俊龙[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [2]长江大学动物科学学院,湖北荆州434025 [3]华中农业大学动物医学院,湖北武汉430070

出　　处：《安徽农业科学》2007年第8期2285-2286,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省科技攻关计划项目(2006AA205A02)

摘　　要：用弓形虫血清抗体阴性的健康家兔,以4种不同的免疫程序皮下接种弓形虫速殖子裂解抗原制备高免血清。研究结果表明,先以完全弗氏佐剂和不完全弗氏佐剂分别进行一免和二免,然后再以抗原进行三免或四免制备高免血清,其抗体效价高达1∶4 096(IHA),与每次都使用抗原的传统免疫程序制备的抗体效价相当,但所用抗原剂量减半,该法优于另外2种免疫程序。Four different immune programs were used to inoculate hypodermically lysis antigen of Toxoplasma gondii tachyzoite and to produce high titer immune serum. The optimum program was selected out： the animal was vaccinated with Freund＇s complete adjuvant at the first time and with Freund＇s incomplete adjuvant at the second time, then vaccinated with antigen at the third and the forth time. The titer of antibody produced by this method increased to 1：4096 （IHA）; meanwhile, the dosage of antigen used for vaccination was minimum.

关 键 词：刚地弓形虫 免疫方法 抗体 

分 类 号：S852.7[农业科学—基础兽医学]