3TSR对胰腺癌的抑制作用及机制探讨  

The inhibitory effects of 3TSR on pancreatic cancer and its mechanism

作　　者：朱少俊[1] 金洲祥[1] 张雪峰[2] 章小平[2] 董磊[3] 徐鲁白[1] 王继生[1] 

机构地区：[1]温州医学院附属第二医院普通外科,温州325027 [2]美国哈佛医学院Beth Isreal Deaconess医学中心外科系 [3]温州医学院病理教研室

出　　处：《中华普通外科杂志》2007年第2期141-144,共4页Chinese Journal of General Surgery

基　　金：浙江省教育厅自然科学基金资助（编号20051188）

摘　　要：目的研究血管形成抑制剂3TSR在体内外对胰腺癌的抑制作用，并初步探讨其机制。方法分别在体外培养条件下比较对照组、3TSR、键择（Gem）和3TSR＋Gem组对胰腺癌MIAPaCa-2细胞增殖及凋亡的影响，以及对裸鼠原位移植瘤肿瘤体积、细胞增殖指数及凋亡的影响。结果体外培养时3TSR对胰腺癌细胞无增殖抑制及诱导凋亡的作用，而Gem对胰腺癌细胞具有明显的抑制增殖及诱导凋亡的作用，但3TSR与Gem在体外无协同作用。体内实验时3TSR组、Gem组和3TSR＋Gem组肿瘤体积较对照组分别缩小70．1％，79．3％和84．9％；3TSR组、Gem组和3TSR＋Gem组胰腺癌细胞增殖指数分别为（19．7±3．1）％，（8．1±1．9）％和（13．2±4．2）％，其中3TSR组增殖指数与对照组比较差异无统计学意义，而Gem组和3TSR＋Gem组细胞增殖指数明显小于对照组和3TSR组（P〈0．05）；3TSR组、Gem组和3TSR＋Gem组胰腺癌细胞凋亡率分别为（5．0±2．2）％，（21．1±4．2）％和（22．8±4．2）％，其中3TSR组胰腺癌细胞凋亡率与对照组比较差异无统计学意义（P〈0．05），而Gem组和3TSR＋Gem组细胞凋亡率显著大于对照组和3TSR组（P〈0．05）；3TSR组及3TSR＋Gem组血管内皮细胞凋亡率又高于对照组和Gem组（P〈0．05）。结论3TSR体内外对胰腺癌细胞无直接增殖抑制及诱导凋亡作用，但能显著减少肿瘤体积，其机制可能与诱导内皮细胞凋亡有关；3TSR与化疗药物Gem合用未明显提高胰腺癌的治疗效果。Objective To investigate the anti-tumor efficacy of angiogenic inhibitor 3TSR on pancreatic cancer cells and to explore its mechanism. Methods We compared the effects of 3TSR, Gem, and 3TSR plus Gem groups on the proliferation and apoptosis of pancreatic cancer cells in vitro. The effects of3TSR, Gem and 3TSR plus Gem groups on tumor volume, cancer cell proliferation, apoptosis and endothelial cell apoptosis were analyzed in pancreatic cancer model of nude mice. Results 3TSR did not inhibit proliferation and nor induce apoptosis of pancreatic cancer cells in vitro. In contrast, Gem significantly inhibited cell proliferation and induced apoptosis. However, the combination of 3TSR and Gem had no synergistic effects. In-vivo studies showed that tumor volume reduced dramatically in 3TSR, Gem and 3TSR plus Gem groups by 70. 1% ,79. 3% and 84. 9%, respectively. The proliferation index of 3TSR, Gem and 3TSR plus Gem groups was （ 19.7 ± 3.1 ） %, （ 8.1 ±1.9） % and （ 13.2 ± 4. 2） %, respectively. The proliferation index was not statistically different between 3TSR and control group, but that of Gem and 3TSR plus Gem group was significantly lower than control group （ P 〈 0. 05 ）. The cancer cell apoptosis rate of 3TSR, Gemand 3TSR plus Gem groups was （5.0±2.2）%,（21.1 ±4.2）% and （22.8 ±4.2）%, respectively. The apoptosis rate was not statistically different between 3TSR and control group, and that of Gem and 3TSR plus Gem group was significantly higher than 3TSR and control group （ P 〈 0. 05 ）. The endothelial cell apoptosis rate of 3TSR and 3TSR plus Gem group was significantly higher than control and Gem group （ P 〈 0. 05 ）. Conclusions 3TSR significantly decreases tumor volume probably by a mechanism of the induction of the apoptosis of endothelial cells; There is no synergistic effects in the combination of 3TSR and Gem.

关 键 词：胰腺肿瘤 血管生成抑制剂 细胞凋亡 内皮细胞 3TSR 

分 类 号：R686[医药卫生—骨科学]