Study on the effects of cefotaxime on intracellular Ca^(2+) in human peripheral lymphocytes by fluoremetry  

作　　者：Dan Dan Wang Hai Yan Wang Ye Hong Zhou Chun Gui Zhao Chuan Dong Shao Min Shuang 

机构地区：[1]School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China [2]Medical College of Shanxi Datong University, Datong 037000, China

出　　处：《Chinese Chemical Letters》2007年第4期424-426,共3页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China （No. 20575038）.

摘　　要：Characteristic of Fura-2-Ca^2＋ interaction was studied based on the fluorescence technique. The apparent dissociation constants （Kd） of the Fura-2-Ca^2＋ complex were determined at different temperature. The effect of cefotaxime （CEFA） on intracellular Ca^2＋ concentration （[Ca^2＋]i） was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca^2＋]i in resting human peripheral lymphocytes was 100 4- 7 nmol/L but after treatment with cefotaxime, the changes of [Ca^2＋]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca^2＋]i increased, as a result of releasing intracellular Ca^2＋ stores. Higher concentration of cefotaxime （50-500 μmol/L） stimulated to decrease of [Ca^2＋]i.Characteristic of Fura-2-Ca^2＋ interaction was studied based on the fluorescence technique. The apparent dissociation constants （Kd） of the Fura-2-Ca^2＋ complex were determined at different temperature. The effect of cefotaxime （CEFA） on intracellular Ca^2＋ concentration （[Ca^2＋]i） was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca^2＋]i in resting human peripheral lymphocytes was 100 4- 7 nmol/L but after treatment with cefotaxime, the changes of [Ca^2＋]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca^2＋]i increased, as a result of releasing intracellular Ca^2＋ stores. Higher concentration of cefotaxime （50-500 μmol/L） stimulated to decrease of [Ca^2＋]i.

关 键 词：FURA-2 LYMPHOCYTES CEFOTAXIME [Ca^2＋]i 

分 类 号：R978.11[医药卫生—药品]