雌激素和孕激素对人乳腺癌细胞MCF-7 RCAS1表达的影响  

Effect of estrogen and progesterone on the expression of RCAS1 in breast cancer cell line MCF-7

作　　者：葛冰磊 洪学军[2] 王青青[2] 

机构地区：[1]宣城市人民医院,安徽宣城242000 [2]浙江大学免疫研究所,浙江杭州310031

出　　处：《检验医学》2007年第2期114-118,共5页Laboratory Medicine

基　　金：国家自然科学基金资助项目(30100163)

摘　　要：目的探讨体外雌激素和孕激素对人乳腺癌细胞MCF-7表达SiSo细胞上的受体结合肿瘤抗原（RCAS1）分子的影响。方法用浓度为10^12～10^-8mol／L的雌激素或浓度为10^-9～10^-5mol／L的孕激素作用乳腺癌细胞MCF-7后，用半定量逆转录聚合酶链反应（RT—PCR）检测MCF-7细胞RCAS1 mRNA水平的变化，用蛋白免疫印迹试验分析MCF-7细胞表达RCAS1蛋白水平的变化。结果在10^-12～10^-8mol／L范围内随着雌激素浓度的增加，MCF-7细胞RCAS1 mRNA的表达呈递增趋势，在10^-11、10^-10、10^-9、10^-8mol／L范围内雌激素组与未处理对照组比较，差异有统计学意义（P〈0．05）。随着10^-9～10^-5mol／L的孕激素浓度的增加，MCF-7细胞RCAS1 mRNA的表达呈下降趋势，孕激素各组与未处理对照组比较，差异有统计学意义（P〈0．05）。用RCAS1单克隆抗体能检出相对分子质量为32000的条带，其灰度随着雌激素浓度的增加而增强，随孕激素浓度的增加而减弱。结论雌激素可上调MCF-7细胞的RCAS1分子表达，孕激素可下调MCF-7细胞的RCAS1分子表达。Objective To study the effect of estrogen and progesterone on the expression of receptor-binding cancer antigen expressed on SiSo cells （RCAS1） in breast cancer cell line MCF-7 in vitro. Methods Human breast cancer cell line MCF-7 cells were exposed to estrogen （ 10^-12-10^-5 mol/L） or progesterone （ 10^-9-106-5 mol/L）, and the expression level of RCAS1 mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） ,then Western blot assay was used to analyze the change of RCAS1 protein expression. Results The expression of RCAS1 mRNA in the estrogen-treated cells was significantly higher than that in the untreated control （ P 〈 0.05）, and the expression level of RCAS1 mRNA increased with the increase of estrogen concentration. The expression of RCAS1 mRNA in MCF-7 treated with progesterone was significantly lower than that in the untreated control （P 〈 0.05） , and the expression of RCAS1 mRNA decreased with the increase of progesterone concentration. A 32 000 band was detected in MCF-7 cell extracts by Western blot assay with the increase of estrogen or progesterone concentration. The bands were detected at a higher or lower intensity compared with untreated control. Conclusions Estrogen can upregulate, while progesterone can down-regulate the expression of RCAS1 both in the level of mRNA and protein.

关 键 词：雌激素 孕激素 MCF-7细胞 SiSo细胞上的受体结合肿瘤抗原 逆转录聚合酶链反应 蛋白免疫印迹试验 

分 类 号：R446.62[医药卫生—诊断学]