2种方法制备丹参酮Ⅱ_A-PLGA纳米粒的比较  

Comparison of two preparation methods applied in tanshinone ⅡA-loaded PLGA nanoparticles

作　　者：甘良春[1] 侯世祥[1] 毕岳琦[1] 王昶光[1] 王新春[1] 陈其翔[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《中国中药杂志》2007年第7期578-581,共4页China Journal of Chinese Materia Medica

摘　　要：目的:考察并优化丹参酮ⅡA-PLGA纳米粒的制备工艺,比较2种制备方法对纳米粒成型及其质量的影响。方法:采用纳米沉淀法和乳化溶剂蒸发法制备纳米粒,并对制得的纳米粒进行质量评价及比较,包括粒径、形态、载药量、包封率、药物利用率、晶型及体外释放行为。结果:沉淀法和乳化溶剂蒸发法制备的纳米粒平均粒径分别为225 nm和183 nm,包封率分别为95.49%和87.99%、载药量分别为2.03%和0.16%、药物利用率分别为38.42%和17.59%。结论:沉淀法制备丹参酮ⅡA-PLGA纳米粒的效果优于乳化溶剂蒸发法。Objective： To optimize formulation of tanshinone ⅡA-loaded PLGA nanoparticles and compare the difference of two methods in preparation and quality of nanoparticles. Method： The two methods were nanoprecipitation method and emulsion-evaporation method. Single factor experiments and central composite design and response surface method were used to optimize the formulation of nanoparticles. The nanoparticles were characterized at size, morphology, entrapment efficiency, drug loading, drug recovery rate, crystallinity and drug release in vitro. Result： The mean diameters were 225 nm and 183 nm, the entrapment efficiency were 95.49% and 87.99%, the drug loading were 2.03% and 0. 16%, and the drug recovery rates were 38.42% and 17.59% respectively for nanoprecipitation method and emulsion-evaporation method. Conclusion： Nanoprecipitation method was better than emulsion-evaporation method for preparation of tanshinone ⅡA-loaded PLGA nanoparticles.

关 键 词：丹参酮ⅡA 纳米粒 沉淀法 乳化溶剂蒸发法 比较 

分 类 号：R284.1[医药卫生—中药学]