刺桐醇提取物对去卵巢大鼠体内钙稳态的影响及作用机制分析  

Effect of Erythrina variegata on Ca^(2+) homeostasis in ovariectomized rats and action mechanism

作　　者：张岩[1] 李晓莉[2] 黄文秀[1] 

机构地区：[1]深圳市中药药学及分子药理学研究重点实验室,广东深圳518057 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2007年第7期627-630,共4页China Journal of Chinese Materia Medica

摘　　要：目的:考察刺桐醇提取物对去卵巢大鼠体内钙稳态的影响及对小肠近端、肾脏相关基因表达的调控。方法:大鼠手术处理4周后,将去卵巢大鼠随机分为模型组、阳性对照组、刺桐低、高剂量组(300,600 mg.kg-1.d-1),另有8只为假手术组,8只为正常对照组。灌胃给药治疗14周。血清、尿液中钙含量采用比色法测定。采用RT-PCR技术分析小肠及肾脏基因表达。结果:对体内钙稳态的影响,与模型组比较,刺桐提取物高、低剂量都显著抑制尿钙排泄(P<0.001),并且有效改善去卵巢大鼠低血钙水平。机制分析表明,刺桐提取物高、低剂量能够成剂量依赖性地显著上调大鼠小肠近端VDR基因表达(P<0.05)以及肾脏CaBP-9k基因表达(P<0.05)。结论:刺桐醇提取物能够维持去卵巢大鼠体内钙稳态。Objective： To study the effect of Erythrina variegata （EV） on Ca^2＋ homeostasis in ovariectomized rats and the regulation on gene expression in duodenum and kidney. Method： Four weeks after surgical operation, the ovariectomized （OVX） rats were administered orally with estradiol and EV extracts for 14 weeks. Ca level in serum and urine was measured by colorimetric methods, and gene expressions in duodenum and kidney were analyzed by reverse transcriptase-polymerase chain reaction （RT-PCR）. Resalt： EV extracts could improve the serum Ca level and inhibite the urinary Ca excretion in OVX rats, and this might be due to the upregulation of EV on VDR mRNA expression in duodenum and CaBP-9k mRNA expression in kidney. Conclusion： EV could maintain Ca homeostasis in OVX rats.

关 键 词：刺桐 去卵巢 钙 基因表达 

分 类 号：R285.5[医药卫生—中药学]