阿米卡星对赤藓红的褪色反应及其分析应用  

Fading Reaction of Amikacin to Erythrosin and Its Analytical Applications

作　　者：安兰香[1] 刘绍璞[1] 谢锂纱[2] 江虹[3] 胡小莉[1] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]四川出入境检验检疫局,成都610041 [3]涪陵师范学院化学系,重庆408003

出　　处：《西南大学学报（自然科学版）》2007年第3期37-40,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20475045);重庆市教委资助项目(KJ051304)

摘　　要：　在酸性介质中，阿米卡星（AMK）与赤藓红（TIF）反应生成离子缔合物，导致赤藓红褪色．其最大褪色波长位于527nm,AMK在0．064-2．500μg／mL浓度范围内遵从比尔定律，表观摩尔吸光系数（ε）为1．46×10^5 L·mol^-1·cm^-1，考察了褪色反应的光谱特征、适宜的反应条件和共存物质的影响．方法具有良好的选择性，用于人体血清及尿样中阿米卡星的测定，结果满意．In an acid medium, amikacin （AMK） reacts with erythrosine （TIF） to form an ion-association complex, which makes the dye fade. The maximum fading wavelength is located at 527 nm..The maximum molar absorptivity ε is 1.46× 10^5 L· mol^-1· cm^-1. AMK follows Beer＇s law in the concentration range of 0. 064-2. 500μg/mL. The spectra characteristics, optimum reaction conditions and the influence of foreign substances have been investigated. The method has good selectivity and has been applied for the determination of the amikacin in human serum samples and urine samples with satisfactory results.

关 键 词：阿米卡星 赤藓红 分光光度法 

分 类 号：O657.32[理学—分析化学]