全程自养脱氮体系中硝化菌株的筛选和鉴定  

Isolation and Identification of Nitro-Bacteria and Nitroso-Bacteria Strains from the Full Autotrophic Ammonium Removeal System

作　　者：黄俊丽[1] 王贵学[1] 肖丽[1] 郭劲松[2] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]重庆大学城市建设与环境学院,重庆400044

出　　处：《西南大学学报（自然科学版）》2007年第1期34-38,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家科技攻关计划(2003BA808A17-02-04).

摘　　要：在建立全程自养脱氮反应器的基础上,以活性污泥为对照,分析了反应器内硝化菌和亚硝化菌的数量变化,并通过富集培养,从反应器中筛选、分离用于治理氮污染的硝化菌和亚硝化菌．研究结果表明,自养脱氮反应器内亚硝化菌数量显著增加,说明亚硝化菌积累是全程自养脱氮体系的一个显著特点．从反应器内富集到参与自养脱氮反应的细菌菌株,鉴定结果表明,参与亚硝化反应的菌株主要为铜绿假单胞菌(Pseudomonas aeruginosa)和食酸丛毛单胞菌(Comamonas acidovorans);参与硝化反应的菌株主要为睾丸酮丛毛单胞菌(Comamonas testoseroni),同时测定了铜绿假单胞菌(Pseudomonas aeruginosa)的亚硝化能力和睾丸酮丛毛单胞菌(Comamonas testoseroni)的硝化能力．The full autotrophic ammonium removal system and activated sludge were compared for their amount of nitro-bacteria and nitroso-bacteria. The bacteria contributing to ammonium removal were enriched and isolated from the full autotrophic ammonium removal system. The number of nitroso-bacteria increased.remarkably in the full autotrophic ammonium removal system, suggesting that the accumulation of nitroso-bacteria is a characteristic feature of the system, Three different bacteria were enriched in the system and two were identified as the nitroso-bacteria Pseudomonas aeruginosa and Comamonas acidovorans, and one as the nitro-bacterium Comamonas testoseroni. The ability of denitrification of P. aeruginosa and the ability of nitrification of C. testoseroni were determined.

关 键 词：全程自养脱氮 硝化菌 亚硝化菌 

分 类 号：Q93[生物学—微生物学]