HPLC-UV检测法测定废水中十二烷基苯磺酸钠  

A High Performance Liquid Chromatography-UV Detection Method for Determination of Sodium Dodecylbenzene Sulfonate in Wastewater

作　　者：莫苹[1,2] 黄玉明[3] 

机构地区：[1]西南大学化学化工学院 [2]三峡库区生态环境教育部重点实验室,重庆400715 [3]重庆市现代分析科学重点实验室

出　　处：《西南大学学报（自然科学版）》2007年第1期65-68,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金(20477033);教育部留学回国人员科研启动基金(教外司留2004-527);重庆市自然科学基金重点资助项目(CSTC2004BA7019).

摘　　要：建立了废水中十二烷基苯磺酸钠的高效液相色谱法(HPLC)分离紫外检测分析方法．探讨了流动相中甲醇含量、电解质种类、电解质浓度、pH及流速等对分离效能的影响,获得了最佳的色谱分析条件:流动相是V_(甲醇): V_水=80:20(含7 mmol/L醋酸铵溶液,pH=9)．在优化的实验条件下,吸光度在5～18000μg/L范围内与十二烷基苯磺酸钠的浓度呈现良好的线性关系．本法的特点是可以在较短时间内用等度方法实现对十二烷基苯磺酸钠的分离和测定,用于废水中十二烷基苯磺酸钠的测定,获得满意结果．A reversed-phase high performance liquid oped for the determination of sodium dodecylbenzene chromatography-UV detection method has bee develsulfonate in wastewater. The effects of methanol content, the type of electrolytes and their concentrations, pH and flow rate in the mobile phase on separation were investigated and optimized. Under the optimized conditions, the absorbance at 254 nm showed a linear relationship with the concentration of sodium dodecylbenzene sulfonate. The proposed method has been applied successfully to the analysis of sodium dodecylhenzene sulfonate in environmental wastewater samples.

关 键 词：十二烷基苯磺酸钠 高效液相色谱法 废水 

分 类 号：X703[环境科学与工程—环境工程]