正交试验法筛选藏药红毛五加多糖的提取分离工艺  

Screening for Optimum Tchnology for Extraction and Isolation of Polysacharides from the Tibentan Traditional Medicine Acanthopanax giraidii Harmas in an Orthogonal Experiment

作　　者：孟庆艳[1] 刘圆[1] 彭镰心[1] 孙卓然[1] 安静[1] 吴远波[1] 

机构地区：[1]西南民族大学少数民族药物研究所,成都610041

出　　处：《西南大学学报（自然科学版）》2007年第1期122-125,共4页Journal of Southwest University(Natural Science Edition)

基　　金：四川省青年基金项目(2007);四川省教育厅教育教学改革项目(2005).

摘　　要：目的:研究藏药红毛五加多糖的提取分离的最佳工艺条件．方法:采用正交设计法筛选．结果:藏药红毛五加多糖的最佳提取分离工艺为:采用加热回流法,石油醚(30～60℃)回流6 h,95%乙醇回流4 h,滤渣加14、12倍量的水,提取2 h,提取2次,提取液浓缩到50 mL,加醇使醇浓度达到80%,静置16 h,减压过滤,滤渣真空干燥,得红毛五加多糖粗品．结论:本实验为藏药红毛五加多糖的提取分离的条件提供了参考．为藏药红毛五加的进一步开发研究提供依据．In an orthogonal experiment, the optimum technological conditions for the extraction and isolation of polysaccharides from Acanthopanax giraidii Marms. were established as follows, circulation with petroleum benzine at 30-60 ℃ for 6 h.→ recirculation with 95% alcohol for 4 h.→ addition of 14- and 12-fold water and extracting for 2 h. （two times）→ concentration to 50 ml. Addition of alcohol until a final concentration of 80% and the product is left undisturbed for 16 h. Crude polysaccharides were obtained after filtration under decompression conditions.

关 键 词：藏药 红毛五加 多糖 提取分离工艺 正交设计 

分 类 号：R931.4[医药卫生—生药学]