新型稀土杂多化合物对乙型肝炎病毒复制的抑制作用  

Inhibitory effect of novel polyoxometalate on hepatitis B virus replication in vitro

作　　者：吴新宇[1] 牛俊奇[2] 吴荻[2] 王宏芳[1] 徐坤[1] 孙志伟[1] 李娟[1] 

机构地区：[1]吉林大学公共卫生学院卫生毒理学教研室,吉林长春130021 [2]吉林大学第一医院感染症科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第2期223-226,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(20171010)

摘　　要：目的:研究稀土杂多化合物(PTW-6)体外抗乙型肝炎病毒(HBV)的活性。方法:MTT法检测PTW-6对Hep G2 2.2.15细胞的毒性,乙型肝炎病毒e(s)抗原诊断试剂盒检测上清液中HBeAg、HBsAg的含量,Southern blotting法检测PTW-6对细胞内HBV DNA复制的抑制作用。荧光定量PCR分析PTW-6对细胞内HBV mRNA和上清液中HBV DNA含量的影响。结果:PTW-6对2.2.15细胞的半数中毒浓度为1590.46 mg.L-1,PTW-6各浓度实验组对HBeAg和HBsAg的抑制率均高于对照组(P<0.05);随着PTW-6浓度的增高,PTW-6对2.2.15细胞内外HBV DNA的抑制率增加,PTW-6对2.2.15细胞外HBV DNA和细胞内HBV mRNA半数抑制浓度分别为51.1和63.6 mg.L-1。结论:PTW-6体外毒性较低,且对HBV复制有较好的抑制作用。Objective To evaluate the activity of novel polyoxometalate against hepatitis B virus （HBV） in vitro. Methods MTT assay was used to evaluate the growth inhibitory effects of polyoxometalate on 2.2.15 cell lines, Hepatitis B e （s） antigen （HBeAg） and Hepatitis B surface antigen in the culture medium were determined by ELISA. Southern blotting was performed to detect HBV DNA in Hep G2 2.2.15 cells. Real-time quantity PCR was applied to detect the contents of introcellular HBV mRNA and extracellular HBV DNA. Results The median toxicity concentration （TC50） was 1590. 46 mg · L^-1 on 2.2.15 cells. The inhibitory rats of PTW-6 on HBeAg and HBsAg were markedly higher compared with control group （P〈0. 05）. The median inhibitory concentration （IC50） of PTE-6 in extracellular HBV DNA and introcellular HBV mRNA were 51.1 and 63.6 mg · L^-1 , respectively.Conclusion Novel polyoxometalate is a potential compound for treatment chronic HBV infection.

关 键 词：稀土杂多化合物 肝炎病毒 乙型 HEP G2 2.2.15细胞 

分 类 号：R512.62[医药卫生—内科学]