采用^(137)CSγ射线辐照灭活红细胞血液制品中大肠杆菌的实验研究  

^(137)Cs gamma irradiation for sterilization of red blood cells contaminated by escherichia Coli

作　　者：黎儒青[1] 蒋天伦[1] 肖瑞卿[1] 林武存[1] 任德会[1] 曾杰[1] 帅桂珍[1] 

机构地区：[1]第三军医大学附属西南医院输血科,重庆400038

出　　处：《中国输血杂志》2007年第1期21-23,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨不同剂量γ射线辐照对红细胞血液制品中大肠杆菌的灭活作用,确定红细胞血液制品灭菌的最佳辐照剂量。方法制备含102-106不同数量级大肠杆菌的红细胞血液制品,应用强度为0、15、20、25、30和35Gy的γ射线进行照射,并于照射后0、7、14d检测血液中大肠杆菌的含量。结果红细胞血液制品经照射后大肠杆菌数量发生了不同程度的改变,当细菌量≤103cfu/ml时,25Gy的γ射线强度足以灭活样本内的所有细菌。结论25Gy的γ射线辐照剂量能有效灭活红细胞血液制品中的细菌,是一种安全有效的红细胞血液制品灭菌剂量。Objective To study the bacteriological effect of different dosages of gamma irradiation on Escherichia Coli （E. Cold in red blood cells （RBC） products,and assess the appropriate dose of gamma irradiation. Methods Prepare RBC samples inoculated with（10^2 - 10^6 ）/ml of E. Coli. The contaminated RBCs were irradiated by 0 （as control）, 15,20,25,30 and 35Gy of gamma irradiation. E. Coil in the RBCs were tested on days 0,7, and 14 after irradiation. Results The quantities of E. Coil changed after irradiation. The RBCs inoculated with less than 10^3cfu/ml of E. Coil were completely sterilized by 25 Gy irradiation. Conclusion 25 Gy gamma irradiation can eradicate less than 10^3cfu/ml of E. Coli in the RBC products.

关 键 词：Γ射线辐照 细菌灭活 移植物抗宿主病 血液保存 

分 类 号：R457.1[医药卫生—治疗学] R457.12[医药卫生—临床医学]