羊草与灰色赖草杂种F_1再生体系的建立  

Study of Plant Regeneration in the Hybrid F_1 Between Leymus chinensis and Leymus cinereus

作　　者：侯建华[1] 张颖[1] 云锦凤[2] 张志 高志军[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010018 [2]内蒙古农业大学生态环境学院,呼和浩特010019 [3]内蒙古伊利实业集团股份有限公司,呼和浩特010080

出　　处：《草地学报》2007年第2期109-112,共4页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(39970536)

摘　　要：对羊草(Leymus chinensis)与灰色赖草(Leymus cinereus)杂种F1幼穗进行离体培养,建立了杂种F1植株再生体系。结果表明:不含任何激素的MS培养基是最佳的分化培养基,分化成苗率为66%;2,4-D对愈伤组织的诱导及其质地的改造有重要作用;3.0 mg/L 2,4-D+MS培养基愈伤组织诱导率最高,为68%;2.0 mg/L 2、4-D+MS诱导的愈伤组织胚性最强;诱导率与分化率没有直线对应关系;再生体系的建立为杂种F1育性恢复的研究奠定了基础。The study was undertaken to induce F1 young spikes between L. chinensis and L. cinereus to develop a system of plant regeneration. Results indicate that hormone-free MS medium was the best in the differentiation at a rate of 66%, while 2,4-D was important for callus induction and quality improvement. The highest rate of callus induction was 68% in the calluses were obtained in the medium of 2.0 mg/L 2,4 callus induction and plant regeneration frequency. The foundation for the fertility restoration in F1. medium of 3. 0 mg/L 2, 4-D＋ MS. Embryogenic D＋MS. There was no lineal correlation between development of the plant regeneration would lay a

关 键 词：杂种F1 幼穗培养 植株再生 羊草 灰色赖草 

分 类 号：Q943[生物学—植物学]