鱼藤酮对体外培养星型胶质细胞毒性作用  

Toxic effects of rotenone on primary cultured astroglia in vitro

作　　者：刘辉[1] 张筱军[2] 吴强[3] 董兆君[1] 

机构地区：[1]第三军医大学毒理学教研室,重庆400038 [2]中国人民解放军第43医院 [3]第三军医大学研究生处

出　　处：《中国公共卫生》2007年第4期411-413,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30400347)

摘　　要：目的观察鱼藤酮对体外培养星型胶质细胞毒性作用的特点。方法体外培养大鼠中脑星型胶质细胞,采用胶质纤维酸性蛋白(GFAP)免疫荧光法鉴定。观察染毒星型胶质细胞形态,检测细胞活力,通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RT-PCR检测缝隙连接蛋白(connexin43,CX43)mRNA表达。结果0.5μmol/L鱼藤酮染毒24 h即可引起明显的细胞形态改变;0.75,1.0,2.0μmol/L鱼藤酮染毒时星型胶质细胞活力明显降低,乳酸脱氢酶释放量显著增加;0.5μmol/L以上鱼藤酮可明显抑制胶质细胞增殖,CX43 mRNA表达降低。结论鱼藤酮对体外培养星型胶质细胞可以产生明显的毒性损伤作用,但与神经元比较,星型胶质细胞对相同剂量的鱼藤酮染毒具有更强的耐受能力。Objective To study the toxic effects induced by rotenone on primary cultured astroglia. Methods Rat astroglia were cultured and identified by glial fibriUary acid protein（GFAP）. MTT assay and morphological observation were used to evaluate the toxicity of rotenone on astroglia, cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RT-PCR. Results Cell morphology was changed after being exposed to 0.5 μmol/L rotenone for 24 hours; the viability of cell cultures was significantly decreased after being treated with 0,75, 1.0 and 2.0 μmol/L rotenone;ceU proliferation was inhibited and the expression of CX43 mRNA was suppressed obviously by 0.5 and 1.0 μmol/L rotenone. Conclusion Dose-dependent toxic effects on primary cultured astroglia could be induced by being exposed to rotenone, but astroglia have higher tolerance to the same dose of rotenone compared with neurons.

关 键 词：鱼藤酮 星型胶质细胞 活力 增殖 

分 类 号：R139.3[医药卫生—劳动卫生]