Apoptin基因诱导A375细胞凋亡信号转导机制  

Study on signal transduction in apoptosis of human melanoma cells A375 induced by apoptin

作　　者：戈全治[1] 赵洪礼[1] 董萍[2] 

机构地区：[1]沈阳军区联勤部疾病预防控制中心,沈阳110034 [2]沈阳军区大连疗养院检验科

出　　处：《中国公共卫生》2007年第4期441-443,共3页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(20042046)

摘　　要：目的研究肿瘤特异性凋亡基因(apoptin)在诱导人黑色素瘤细胞A 375凋亡中的信号转导机制。方法用含有apoptin基因的真核表达载体瞬间转染体外培养的人黑色素瘤细胞A 375;采用RT-PCR、DNA凝胶电泳、流式细胞仪分析检测A 375的细胞的凋亡;以比色法检测半胱天冬蛋白酶8(caspase-8)和caspase-3的相对活性。结果Apoptin基因瞬间传染的A 375细胞可出现典型的细胞凋亡所具有DNA梯状带;流式细胞术发现实验组细胞凋亡率明显高于其他各组(P<0.01);Cas-pase-3的活性升高,但caspase-8活性没有明显变化。结论Apoptin基因可通过激活caspase-3诱导人黑色素瘤细胞A 375凋亡。Objective To study whether apoptin gene induces apoptosis in human melanoma cells A 375 via the activation caspase - 8 and caspase - 3. Methods The human melanoma cells A 375 were transiently transfected with recombinant pcDNAA 3 plasmid, which contained apoptin gene, and the apoptosis was measured by DNA agarose electrophoresis, RT - PCR and flow cytometry. Caspase - 8 and caspase - 3 relative activity was analyzed by colorimetric assay at various time. Results The apoptin gene could induce apoptosis of human melanoma cells A 375in vitro and the mRNA of apoptin gene could be detected by RT - PCR. The activity of caspase- 3 began to rise in A 375 cells after transfected with apoptin gene, but that of caspase - 8 did not change. Conclusion Apoptin gene can induce apoptosis in human melanoma cells A 375 via active caspase - 3.

关 键 词：肿瘤特异性凋亡基因 细胞 凋亡 半胱天冬蛋白酶 

分 类 号：R459.9[医药卫生—治疗学]