汞中毒大鼠脊髓GFAP表达与药物干预研究  

Study on spinal cord GFAP of mecury poisoning rat and drug trial

作　　者：周永田[1] 徐旭东[1] 刘正亮[1] 谢林[1] 秦占霞[1] 马娟[1] 史春霞[1] 沙海洋[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院康复理疗科,济南250002

出　　处：《中国公共卫生》2007年第4期474-476,共3页Chinese Journal of Public Health

基　　金：山东省医科院2004年科研项目(2004-0023)

摘　　要：目的观察HgCl2对大鼠脊髓星形胶质细胞的影响。方法SD大鼠30只,雌雄各半,体重160~200 g。随机分为3组,每组10只。大剂量组按17 mg/kg（1/4 LD50）经口灌胃HgCl2溶液,1次/d。小剂量组按8.5 mg/kg（1/8 LD50）经口灌胃HgCl2溶液,1次/d。对照组经口灌胃生理盐水2 ml,1次/d,3组均连续灌胃（20±2）d。染汞组建成亚急性汞中毒模型后,各组均处死5只雄鼠,留5只雌鼠作药物干预试验。染汞组用二巯丙磺钠注射液（DMPS）按28 mg/kg腹腔注射驱汞2个疗程,小剂量组仅用DMPS,大剂量组在用DMPS的期间内,按29.22/（kg.d）经口灌胃己酮可可碱（POF）溶液14 d。实验结束后处死全部动物,灌注固定取出脊髓L5-6节并制成病理切片。用免疫组化链酶亲和素-生物素-过氧化物酶聚合物（SABC）法测定脊髓胶质纤维酸性蛋白（GFAP）。结果大、小剂量组GFAP平均灰度均显著低于对照组,阳性细胞率均显著高于对照组。DMPS＋POF显著降低GFAP阳性细胞率,提高平均灰度值,单纯应用DMPS无此作用。结论亚急性HgCl2中毒大鼠脊髓GFAP表达上调,POF能够有效抑制星形胶质细胞激活。Objective To observe the effect of HgCl2 on the astroeyte in spinal cord. Methods Thirty SD rats（15♀, 15 ♂, weighting 160 -- 200g） were randomized into 3 groups of 10（5 ♀, 5 ♂ ） each. Group A and group B received HgCl2 juice at a dose of 17mg/kg（ 1/4 LD50）, 8.5 mg/kg（ 1/8 LD50） respectively by gavage daily. Group C received 0.9 % saline 2 ml by garage daily. To found the sub- acute mercury poisoning rat model within（20 ± 2） days. Then male rats in each group were dissected and the chelator（DMPS） was injected into the abdomen of female rats of group A and B at a dose of 28 mg/kg for 2 periods . Group A received DMPS＋ POF（pentoxifylline） and group B received DMPS only. The POF was used at a dose of 29.22 mg/kg by garage daily for 14 days . The rats were all dissected and the spinal cord （L5 - 6） were gotten after the trial finished. Then the pathological section of spinal cord was made from and GFAP was detected . Results The average gray scale in group A and group B are all less and the positive cell rate are all larger signlfieantly compaired with control group. The POF depress the activation of astrocyte significantly. Conclusion Sub - acute poisoning of HgCl2 enhance GFAP level significantly in spinal cord, and POF depresses the activation of astrocyte.

关 键 词：汞中毒 神经病理性疼痛 脊髓 星形胶质细胞 胶质纤维酸性蛋白 

分 类 号：R135.13[医药卫生—劳动卫生]