人参皂苷-Rh2衍生物体外诱生小鼠细胞因子的作用及对NO水平的影响  

Effect of ginsenoside-Rh2 with molecular modification on secretion of cytokines and NO level in mouse in vitro

作　　者：郭志廷[1] 伊鹏霏[1] 禇秀玲[1] 王鲁[1] 韦旭斌[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《畜牧与兽医》2007年第1期1-3,共3页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30471272)

摘　　要：探讨了经分子修饰后的人参皂苷-Rh2(衍生物)对小鼠脾T淋巴细胞分泌IL-2与IFNγ-、腹腔巨噬细胞分泌TNF-α与NO水平的影响。应用双抗体夹心ELISA法测定3种细胞因子的含量,硝酸还原酶法测定NO的含量。结果表明,衍生物的3个浓度(100,200和300μg/mL)均抑制了IL-2(P<0.01)和NO(P>0.05)的分泌,明显促进了IFN-γ和TNF-α的分泌(P<0.01)。提示衍生物可能是通过细胞因子途径来调节机体的免疫功能。Contents of IL-2 and IFN-γ secreted by spleen T lymphocyte and TNF-α and NO secreted by peritoneal macrophage from mice induced by ginsenoside -Rh2 with molecular modification （the derivate） were determined by DAS-ELISA or by nitrate reductase approachs. The results showed that secretion of IL-2 （P 〈0.01 ） and NO （P 〉0. 05 ） were inhibiteded, and secretion of IFN-γ and TNF-α were significantly enhanced by the derivate at concentrations ranging from 100μg/mL to 300μg/mL （P 〈0.01 ）, suggesting that the derivate could regulate mouse immunologic function by the way of cytokines.

关 键 词：人参皂苷-Rh2 细胞因子 分子修饰 衍生物 

分 类 号：S858.912.4[农业科学—临床兽医学]