雷公藤单体T4对大鼠变态期精子细胞核蛋白转换的影响  

The Effect of Tripterygium Wilfordii Monomer T4 on RatSpermatid Nuclear Protein Transition

作　　者：费仁仁[1] 陈晖[1] 戴文平 刘平 陈啸梅 薛社普 

机构地区：[1]中国医学科学院药物研究所,中国医学科学院基础医学研究所

出　　处：《生殖与避孕》1996年第1期46-48,T003,共4页Reproduction and Contraception

摘　　要：大鼠喂服雷公藤单体Ｔ４７周后，取睾丸分离长形精子细胞核，取附睾分离精子核，提取总碱性核蛋白（ＴＮＢＰ），经电泳扫描后分析，发现长形精子细胞核ＴＨ／ＲＰ（总组蛋白／大鼠精核蛋白）比值升高，糖核蛋白含量下降，附睾精子核碱性蛋白也发生类似的改变，表明睾丸变态期精子细胞组蛋白一精核蛋白取代反应受阻，进而导致附睾精子核蛋白异常，这可能是Ｔ４导致大鼠不育的重要原因。文中还对精核蛋白与生育力的关系予以讨论。Rat testis elongating spermatids and epididymal sperms were collected after 7 weeks of treatment with Tripterygium Wilfordii monomer T4. Total nuclear basic protein (TNBP) were extracted from the elongating spermatid nuclei and the sperm nuclei isolated by sonication. Polyacrylamide gel electrophoresis has been used to separate the TNBP and individual proteins were quantified by scanning microdensitometry. It was found that the content of protamine was reduced and the TH (Total Histones ) /RP (Rat Protamines ) ratios were increased following treatment in the testis elongating spermatids and the same result was found in the epididymal sperms. These results suggest that the interruption of nuclear protein transition of testis spermatids induced by T4 might cause aberrant epididymal sperm nuclear protein and lead to infertility. The relationship between protamine and fertility was discussed.

关 键 词：雷公藤 抗生育作用 精子细胞 核蛋白 

分 类 号：R979.21[医药卫生—药品] R286.921[医药卫生—药学]