家兔输精管结扎后睾丸雄激素受体mRNA表达及与血浆睾酮变化关系的研究  

Study on Testicular ARmRNA ExPression and Changesof Plasma Testosterone in Vasectomized Rabbits

作　　者：李杨[1] 赵丹[1] 王洪军[1] 王蔚东[1] 田庚[1] 赵雪俭[1] 

机构地区：[1]白求恩医科大学生殖病理生理研究室

出　　处：《生殖与避孕》1996年第1期65-69,T004,共5页Reproduction and Contraception

摘　　要：３０只日本大耳白雄兔，分为输精管结扎２５月组（ＶＧ２５）和假手术２５月组（ＳＯＧ２５），输精管结扎６月组（ＶＧ６）和假手术６月组（ＳＯＧ６）。应用放射免疫技术测定血浆睾酮含量；应用大鼠雄激素受体（ＡＲ）ｃＤＮＡ探针，Ｎｏｒｔｈｅｒｎ杂交方法检测睾丸组织的ＡＲｍＲＮＡ表达，分析输精管结扎对睾丸ＡＲｍＲＮＡ表达强度的影响。结果表明（１）血浆睾酮含量以ＶＧ６为最低（８．０６±２．７８ｎｍｏｌ／Ｌ），但与ＳＯＧ６（１０．６８±０．９９ｎｍｏｌ／Ｌ）比较，Ｐ＞０．０５．ＶＧ２５（１２．１０±２．４４ｎｍｏｌ／Ｌ）略高于ＳＯＧ２５（１０．９３±１．２８ｎｍｏｌ／Ｌ），并显著地高于ＶＧ６（ｔ＝３．０９，Ｐ＜０．０１）。（２）睾丸组织ＡＲｍＲＮＡ的Ｎｏｒｔｈｅｒｎ杂交分析证明，家兔睾丸组织的ＡＲｍＲＮＡ与大鼠有同源性，杂交信号位于２８ｓ之后。在ＶＧ６、ＳＯＧ６、ＶＧ２５和ＳＯＧ２５均有ＡＲｍＲＮＡ的表达，ＶＧ６强于ＳＯＧ６，ＶＧ２５与ＳＯＧ２５无明显差异。该结果显示了睾酮对ＡＲｍＲＮＡ表达的下调节作用，而输精管结扎对家兔ＡＲｍＲＮＡ的长度无影响。Thirty male Japan white rabbits were divided into four groups:Vasectomy Groups in 6 and 25 months (VG6 .VG25) and the corresponding Sham Operation Groups (SOG6.SOG25).Plasma testosterone content (T) was detected by RIA ; Androgen receptor (AR) mRNA concentrations were determined by Northern blotting with rat AR cDNA probe. The results showed that: (1) Tin VG6 (8. 06 ±2. 78nmol/L) was the lowest in four groups,but compared with in SOG6 (10. 68 ±0. 99nmol/L) P > 0. 05; T in VG25 (12. 10 ±2. 44nmol/L) was slightly higher than that in SOG25 (10. 93± 1. 28nmol/L) and significantly higher than that in VG6 as well (t= 3. 09,P<0. 01). (2) AR mRNA of rabbit testis showed a homology with rat. The major AR mRNA species was behind 28s, and although ARmRNA expression was detected in VG6. SOG6. VG25 and SOG25, concentration of AR mRNA was the highest in VG6 and the lowest in VG25. Taken together, these data demonstrated that androgen down regulated the concentration of AR mRNA in testis;no changes were found in the length of AR mRNA.

关 键 词：输精管结扎术 睾丸 雄激素 受体 mRNA 睾酮 兔 

分 类 号：R699.8[医药卫生—泌尿科学]