马尾松随机扩增多态性DNA标记的分离研究  

SEGREGATION ANALYSIS OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER IN PINUS MASSONIANA

作　　者：魏令波[1] 唐谦[1] 郑先武[1] 顾万春[1] 李小兵[1] 

机构地区：[1]中国林业科学研究院林业研究所,中国科学院遗传研究所植物生物技术开放实验室

出　　处：《林业科学》1996年第5期476-480,T001,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金;国家"八五"攻关专题

摘　　要：马尾松随机扩增多态性ＤＮＡ标记的分离研究魏令波，唐谦，郑先武，顾万春（中国林业科学研究院林业研究所北京１０００９１）李小兵（中国科学院遗传研究所植物生物技术开放实验室北京１０００１２）关键词马尾松，ＲＡＰＤ，Ｍｅｎｄｅｌｉａｎ分离随机扩增多态性ＤＮＡ...A study on the allelic nature of random amplified polymorphic DNA (RAPD) Marker was made with femalegametophytes of half-sib seeds from single tree of Masson's Pine. On the basis of screening the primers for amplification effects, the Mendelian 1: 1 segregation was tested for amplified products of 40 seeds from C-16 tree of Zhong Yi population with 17 primers. The results showed that the number of amplified fragments for each primer ranged from 7-22, and the total number of amplified fragments over 17 primers was 201. Among these, there were 129 heterozygous bands which occupied 64 percent of total fragments. Through χ2 test (P<0. 05), 55 fragments (Loci) fit the 1: 1 segregation of Mendelian, which were 27 percent of total amplified fragments, These research results will provide the Mendelian RAPD marker for testing genetic variation analysis of single tree or populations and linekage map construction in Masson's Pine.

关 键 词：马尾松 随机扩增多态性 DNA 遗传标记 

分 类 号：S791.248[农业科学—林木遗传育种] Q31[农业科学—林学]