白腐真菌对染料脱色的培养条件研究  

Studies on culture conditions of decolorizaion by Panus conchatus

作　　者：常天俊[1] 潘文维[1] 赵丽[1] 胡道伟[2] 杨英[1] 

机构地区：[1]河南理工大学资源环境学院,焦作454000 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《环境工程学报》2007年第2期54-58,共5页Chinese Journal of Environmental Engineering

基　　金：河南理工大学青年基金资助项目(Z050401)

摘　　要：对贝壳状革耳菌高产漆酶条件及其对染料脱色条件进行了研究。结果得到高产漆酶的最佳条件为:葡萄糖10 g/L,酒石酸铵0.5 g/L,pH 3.0,转速120 r/m in;脱色效果最佳的培养条件是:葡萄糖10 g/L,酒石酸铵0.5 g/L,pH 3.0,温度30℃。由此可知漆酶在染料脱色中起重要作用;实验也表明:随着培养基中碳源、氮源量的增加,菌体生物量逐渐增加,但体系中高碳氮比对菌体对染料的脱色是不利的;菌体生物量与对染料的脱色作用不呈正相关。The optimum conditions for producting laccase and decoloring dyes by Panus conchatus were studied. The results showed that the optimum culture conditions were gulcose 10 g/L,ammonium tartrate 0.5 g/ L, pH 3, temperature 30 ℃. It was the same to the optimum culture of producting laccase by Panus conchatus, and the decolorizaiton rate was positively correlative with the laccase activity. With the increasing concentration of carbon and nitrogen sources, there was a corresponding increase of the biomass. However,the decolorization rate was not related to the biomass of panus conchatus directly.

关 键 词：贝壳状革耳菌 染料 脱色 漆酶 

分 类 号：Q7[生物学—分子生物学]