肿瘤坏死因子α与下肢静脉性溃疡发病的相关性研究  

The correlation between tumor necrosis factor-α and venous ulceration of the lower limbs

作　　者：刘大钺[1] 王深明[1] 钱结胜[2] 叶洁莲[1] 黄雪玲[1] 

机构地区：[1]中山大学附属第一医院血管外科,广州510080 [2]中山大学附属第三医院血管介入中心

出　　处：《中华普通外科杂志》2007年第3期215-217,共3页Chinese Journal of General Surgery

摘　　要：目的 探讨肿瘤坏死因子α（TNF-α）与慢性静脉功能不全时（CVI）下肢静脉性溃疡发病的相关性。方法 采用酶联免疫吸附试验（ELISA）和原位杂交的方法检测CVI伴有皮肤溃疡（10例），CVI不伴有皮肤溃疡（10例）及8例健康自愿者立位及平卧位时下肢静脉血及局部皮肤中TNF-α表达。结果相同体位时，溃疡组患肢血液和皮肤中TNF-α升高，与无溃疡组和对照组相比，差异有统计学意义（P〈0．05）；无溃疡组与对照组相比，差异无统计学意义（P〉0．01）；体位改变并没有引起TNF-α的显著变化（P〉0．05）。结论 TNF-α高表达可能与下肢静脉性溃疡发生具有密切的相关性。Objective To investigate the correlation between TNF-α and venous ulceration of the lower limbs in patients of chronic venous insufficiency （CVI）. Methods CVI cases were divided into ulceration group（ 10 cases）, non- ulceration group （10 cases） and 8 healthy volunteer served as control. TNF-α was analyzed by ELISA and hybridization in situ when patients and controls were posed in different body positions. Results In the same position, TNF-α in the ulceration group was higher than that in the two other groups （P 〈 0. 05 ）, the difference was not significant between non-ulceration and control group. TNF-α level didn＇t change significantly with position. Conclusion The correlation between high expression of TNF-α and venous ulceration of lower limbs is significant.

关 键 词：肿瘤坏死因子Α 酶联免疫吸附测定 原位杂交 静脉性溃疡 

分 类 号：R686[医药卫生—骨科学]