I─B4阳性纤维和终末及P物质受体在大鼠腰骶髓内的分布  

THE DISTRIBUTION OF I B 4 LIKE FIBERS AND SPR LI IN THE RAT LUMBOSACRAL SPINAL CORD

作　　者：郑恒兴[1] 吕岩[1] 秦秉志[1] 李继硕[1] 

机构地区：[1]第四军医大学解剖学教研室 脑研究中心

出　　处：《解剖学报》1996年第4期378-382,T007,共6页Acta Anatomica Sinica

摘　　要：为了探讨传递伤害性刺激信息的Ｃ纤维终末与ＳＰ受体（ＳＰＲ）阳性结构间的关系，用Ｉ－Ｂ４组织化学法和ＳＰＲ免疫组织化学法，光镜下观察了大鼠腰骶髓内Ｉ－Ｂ４阳性纤维及其终末和ＳＰＲ阳性胞体及其树突的分布。结果显示：被Ｉ－Ｂ４特异标记的阳性纤维及其终末主要分布于腰骶髓后角Ｉ、ＩＩ层以及内、外侧束。另外，在腰骶髓的后连合核区（ＤＣＮ）也有相当数量的Ｉ－Ｂ４阳性纤维及其终末分布。说明了伤害性刺激信息主要传递至腰骶髓内的后角Ｉ、ＩＩ层，同时也有相当一部分伤害性刺激信息传递至腰骶髓的ＤＣＮ区。传递至ＤＣＮ区的Ｉ－Ｂ４阳性纤维主要通过沿后角内侧缘走行的内侧束，从而为ＤＣＮ区参与内脏伤害性刺激信息传递及其调制机制提供了进一步的证据。ＳＰＲ阳性胞体和树突主要分布于腰骶髓的后角Ｉ层、Ｉ～Ⅳ层，后角侧缘的内、外侧束也有少量分布。此外，ＤＣＮ区也有大量的ＳＰＲ阳性树突和散在的阳性胞体。双重反应的结果显示两者在Ｉ、ＩＩ层及ＤＣＮ区的分布相互匹配。andeiraea Simplicifolia Isolectin B 4(I B 4) is a specific marker of unmyelinated primary afferent C fibers and terminals. I B 4 histochemical method was used to investigate the distribution of C fibers and terminals in the rat lumbosacral spinal cord. The results showed that I B 4 like fibers and terminals locate in lamina I and II, medial and lateral tracts, and the dorsal commissural nucleus (DCN) of the rat lumbosacral spinal cord. This indicated that the nociceptive information was transmited not only to lamina I and II but also to DCN. The I B 4 like fibers arrived to DCN by medial tracts. And this suggested that the I B 4 like fibers of DCN might transmit the nociceptive information from viscera. We also investigated the distribution of SP receptors (SPR) in the rat lumbosacral spinal cord using immunohistochemical method. The results showed that dendrites of SPR like immunoreactive (SPR LI) products were mainly observed in lamina I and DCN, and partly in laminae II to Ⅳ. The SPR LI neuronal somatos were scattered in this areas. Double labeling of SPR LI and I B 4 like fibers indicates that the distributions of SPR LI and I B 4 like fibers matched quite well. This suggests that some of the primary afferent C fibers and terminals may be substance P like fibers and terminals.

关 键 词：C纤维 P物质 受体 腰骶髓 后连合核 组织化学法 

分 类 号：R323.43[医药卫生—人体解剖和组织胚胎学] R329.4[医药卫生—基础医学]